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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2017.tde-19012017-115607
Document
Author
Full name
Francielli Vilela Peres
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Pellizari, Vivian Helena (President)
Nakayama, Cristina Rossi
Sumida, Paulo Yukio Gomes
Title in Portuguese
Diversidade e conectividade de comunidades bacterianas em substratos sintéticos e orgânicos no atlântico sudoeste profundo.
Keywords in Portuguese
Biofilme
Mar profundo
Oceano atlântico
Abstract in Portuguese
Organismos de mar profundo encontram limitações na disponibilidade de alimentos e exploram enriquecimentos orgânicos esporádicos que chegam ao assoalho oceânico. O objetivo deste trabalho foi descrever a diversidade das comunidades bacterianas associadas a parcelas sintéticas e orgânicas (vértebras de baleia e blocos de madeira) no Espírito Santo, Rio de Janeiro e São Paulo a 3.300 m de profundidade, avaliando a influência dos substratos e da localização geográfica sobre essas comunidades. Foi realizada a extração de DNA e amplificação do gene RNAr 16S para sequenciamento por Illumina Miseq e análises estatísticas pelo Qiime. Os Gêneros dominantes nos substratos sintéticos, madeira e vértebras foram Psychroserpens (Flavobacteriia), Phaeobacter, (Alphaproteobacteria), Desulfobacter, (Deltaproteobacteria), respectivamente. Com base nos resultados obtidos, afirma-se que o tipo de substrato teve maior influência do que a localização geográfica sobre a estrutura das comunidades bacterianas.
Title in English
Diversity and connectivity of bacterial communities in synthetic and organic substrates in the deep southwest atlantic.
Keywords in English
Atlantic ocean
Biofilm
Deep-sea
Abstract in English
Deep sea organisms found limitations in the availability of food and exploit sporadic organic enrichments that reach the ocean floor. The aim of this study was to describe the diversity of bacterial communities associated with synthetic and organic substrate (whale bone and wood blocks) in Espírito Santo, Rio de Janeiro and Sao Paulo to 3,300 m deep, assessing the influence of substrates and location geographical about these communities. 16S rRNA sequencing was performed by Illumina Miseq and statistical analysis by Qiime. The dominant genera in synthetic substrates, wood and vertebrae were Psychroserpens (Flavobacteriia), Phaeobacter (Alphaproteobacteria) and Desulfobacter, (Deltaproteobacteria), respectively. Based on these results, it is stated that the substrate type had greater influence than geographic location on the structure of bacterial communities.
 
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Publishing Date
2017-01-19
 
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