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Master's Dissertation
DOI
10.11606/D.99.2017.tde-10052017-080240
Document
Author
Full name
Cecilia de Oliveira Lavitschka
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Galati, Eunice Aparecida Bianchi (President)
Andrade, Andrey José de
Braz, Lucia Maria Almeida
Title in Portuguese
Competência vetora de Nyssomyia intermedia e Nyssomyia neivai (Diptera, Psychodidae, Phlebotominae) para Leishmania (Viannia) braziliensis
Keywords in Portuguese
Entomologia médica
Leishmania braziliensis
Leishmaniose cutânea
Abstract in Portuguese
Leishmania braziliensis é o agente responsável pela maior parte dos casos de leishmaniose tegumentar e mucocutânea no Brasil. Entre as espécies incriminadas como vetoras, na região Sudeste encontram-se Nyssomyia intermedia e Nyssomyia neivai. A comprovação destas espécies como responsáveis pela transmissão de Leishmania sp. depende de vários fatores, entre estes, a competência vetorial, definida como a capacidade da espécie se infectar e repassar a infecção para um hospedeiro vertebrado. Objetivo: O presente estudo teve como objetivo avaliar experimentalmente a competência vetorial de Ny. intermedia e Ny. neivai para transmitir L. braziliensis. Métodos: Fêmeas de primeira geração de ambas as espécies foram alimentadas em hamster infectado com L. braziliensis e após a oviposição, desafiados a um segundo repasto sanguíneo em hamsters susceptíveis (limpos). Adicionalmente, foram obtidos dados do desenvolvimento imaturo das duas espécies. Resultados: em relação ao ciclo imaturo, observou-se que a maior mortalidade ocorreu no primeiro instar larval para ambas as espécies, 27,6% para Ny. intermedia e 53,9% para Ny. neivai. O quarto instar larval teve a maior duração, com mediana de 8 a 12 dias. Quanto à susceptibilidade à infecção das fêmeas colonizadas de ambas as espécies 0,89% das fêmeas de Ny. intermedia e 8,33 % das fêmeas de Ny. neivai apresentaram infecção por L. braziliensis. Nenhuma fêmea de Ny. intermedia realizou um segundo repasto sanguíneo, enquanto que 3 fêmeas de Ny. neivai realizaram. Destas, duas estavam infectadas e o hamster potencialmente infectado ficou em observação por dois meses. Amostras de tecido do hamster exposto demostraram, por meio de PCR, a presença de L. braziliensis. Conclusão: Nyssomyia intermedia apresentou desenvolvimento de formas flageladas porém não repassou a infecção a um hospedeiro suscetível. Nyssomyia neivai, além de se infectar com a L. braziliensis, também repassou a infecção a um hospedeiro suscetível, demostrando assim sua competência vetora.
Title in English
Vector competence of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae, Phlebotominae) for Leishmania (Viannia) braziliensis
Keywords in English
Cutaneous leishmaniasis
Leishmania braziliensis
Medical entomology
Abstract in English
Leishmania braziliensis is the agent related to cases of cutaneous and mucocutaneous leishmaniasis in Brazil. Among the sandflies involved in the Leishmania sp transmission in the Southeast Region, Ny. intermedia and Ny. neivai are suspected vectors. However, the incrimination of these species as vectors in the transmission of Leishmania sp. depends of several factors, including vector competence, defined as the ability of the species to develop the agent and transmit it to a vertebrate host. Objective: The present study aimed to demonstrate the vector competence to Ny. intermedia and Ny. neivai for L. braziliensis. Methods: To develop the experiments,specimens of both species were reared under laboratory conditions and the adults obtained were used to evaluate the vector competence. Thefemales were fed in infected hamsters and after oviposition, challenged to take a bloodmeal in suscetible hamsters (without infection). All females fed on infected hamster were dissected. The hamster bitted in the exposition were follow up for two months, after that, were euthanatized to evaluate the infection by PCR. Result: The highest immature mortality occurred in the first instar larval for both species, 27.6% for Ny. intermedia and 53.9% for Ny. neivai. The fourth larval instar had the longest duration, with a median varying between 8 to 12 days. About the females of Ny intermedia, only 0.89% of them develop the flagellates. For Ny. neivai, among 36 females feed on infected hamster, 2 were infected. None female of Ny intermedia take a second blood meal and 3 of Ny. neivai have had. The hamsters bitted by the infected females were follow up for 60 days. After that the hamster were euthanatized and the infection was observed. Conclusion: The vector competence of Ny. neivai to transmit Leishmania braziiensis was demonstrated. The susceptibility of Ny. intermedia to develop this leishmania was corroborated.
 
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Publishing Date
2017-05-15
 
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