Rheumatic mitral stenosis (MS) is one of the most frequent manifestations of rheumatic carditis. It is still an important cause of heart disease in young people around the world with higher prevalence in developing countries. Recently, two-dimensional strain using the Speckle tracking (ST) technique, as well as the analysis using three-dimensional echocardiogram (3D ECO), have been incorporated into traditional echocardiography to allow more in-depth study of atrial and ventricular function in several pathologies, including in patients with rheumatic MS. However, despite these advances, studies comparing the assessment of atrial and right ventricular function with two-dimensional methods of assessing severity as well as with pro-BNP NT in patients with rheumatic MS are still scarce. OBJECTIVES: To correlate and establish sensitivity, specificity and accuracy of echocardiographic assessment of left atrial function and right ventricle, using the two-dimensional ST technique as well as by 3D ECO with echocardiographic, clinical and laboratory parameters of severity in patients with rheumatic MS. METHODS: This is an analytical, observational and cross-sectional study, consisting of 61 patients with isolated rheumatic MS in sinus rhythm and no previous interventionist treatment. Patients underwent clinical examination, electrocardiogram and 3D ECO, as well as dosage of NT pro-BNP. RESULTS: The value of R 15% ± 7.6, FATAE 31% ± 12 and the SGL of the RV of - 20% ± 5 and the mean FEVD by ECO 3D was 47% ± 7, A strong positive correlation was observed between the R of AE, and FETAE and AV ( = -0.735; p <0.001, and = 0.661; p <0.001 respectively) and moderate negative correlation SGL VD and FEVD ( = 0.592; p <0.001, = 0.425 p = 0.001 , = 0.447; p = 0.001 respectively). A strong negative correlation was observed between the AE AR and NT-pro BNP ( = -0.695; p <0.001 and = -0.620; p <0.001 respectively). The cutoff values for discriminating patients with important MS by R was 18.4% (sensitivity of 92,8% and specificity of 76,4% AUC = 0.90; p 19 <0.001), SGL VD was 19,2% (sensitivity of 58,9% and specificity of 88,2%, AUC = 0.69; p = 0.019), of FETAE was 31% (sensitivity of 72% and specificity of 100%, AUC = 0, 90; p <0.001). NT pro-BNP was 120pg / dL (sensitivity of 92,8% with specificity of 60% AUC = 0.78; p = 0.001). Only ?R and mean transmittal GD were those that behaved as independent variables (p <0.001). CONCLUSION: The R, FETAE and FEVD 3D correlate with the severity of MS, as well as elevated NT-pro BNP values. RV SGL correlated with severity, but not with NT-pro BNP values.