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Master's Dissertation
DOI
Document
Author
Full name
Bruno Dal Rovere Contesini
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Lorena, 2019
Supervisor
Committee
Guimarães, Daniela Helena Pelegrine (President)
Avila, Ivonete
Canettieri, Eliana Vieira
Prata, Arnaldo Márcio Ramalho
Title in Portuguese
INVESTIGAÇÃO DA INFLUÊNCIA DE PARÂMETROS LUMINOSOS NO DESENVOLVIMENTO DE CULTIVOS DA MICROALGA MARINHA Chlorella minutíssima
Keywords in Portuguese
Chlorella minutíssima
Biofotônica
Biomassa
Lipídios
Microalgas marinhas
Planejamento de experimentos
Taguchi
Abstract in Portuguese
O presente trabalho compreende a execução de 16 experimentos, realizados em duplicata, visando avaliar a influência de fatores, tais como iluminância, concentração de NaH2PO4.H2O, concentração de NaNO3, fotoperíodo e comprimento de onda, além da interação entre a iluminância e os demais fatores, na produção de biomassa e lipídios pela microalga marinha Chlorella minutíssima. Os experimentos estiveram dispostos em 2 arranjos ortogonais L8 de Taguchi, cada um dos quais recebeu dados obtidos em experimentos com diferentes comprimentos de onda (470 nm, além da luz branca), nos quais eram variados, em dois níveis, a iluminância (26 klx e 13 klx), as concentrações de NaH2PO4.H2O (5 mg/L e 15 mg/L) e NaNO3 (45 mg/L e 75 mg/L) e o fotoperíodo (12: 12 horas claro: escuro e 8 ciclos diários de 2: 1 horas claro: escuro). Os experimentos se processaram em reatores de vidro com formato de prisma de base quadrada com volume útil de 1,1 L, isolados da influência da luminosidade externa. Para a luz branca, o melhor resultado para a produção de biomassa foi de 1300 mg/L, ao passo que o melhor resultado para o teor lipídico foi de 22,75% em relação à biomassa seca. Para a luz de comprimento de onda equivalente a 470 nm, o melhor resultado para a produção de biomassa foi de 1270 mg/L, ao passo que o melhor resultado para o teor lipídico foi de 19,03% em relação à biomassa seca.
Title in English
Investigation of the incluence of luminous parameters on the development of marine microalgae Chlorella minutíssima
Keywords in English
Chlorella minutíssima
Biomass
Biophotonics
Design of experiments
Lipids Marine microalgae
Taguchi
Abstract in English
This work comprises the execution of 16 experiments, performed in duplicate, in order to evaluate the influence of factors such as illuminance, NaH2PO4.H2O concentration, NaNO3 concentration, photoperiod and wavelenght, in addition to the interaction between the illuminance and the others factors, in the production of biomass and lipids by the m arine microalgae Chlorella minutíssima. The experiments were arranged in 2 Taguchi's L8 orthogonal arrays, each of which received data obtained in experiments with different wavelengths (470 nm, in addition to the white light), in which the illuminance (26 klx and 13 klx), the NaH2PO4.H2O (5 mg/L and 15 mg/L) and NaNO3 (45 mg/L and 75 mg/L) concentrations and the photoperiod (12: 12 hours light: dark and 8 daily cycles of 2: 1 hours light: dark) were varied, in two levels. The experiments were performed in quadrangular prism glass reactors with a useful volume of 1.1 L, isolated from the influence of external luminosity. For white light, the best result for biomass production was 1300 mg/L, while the best resulto f lipid contente was 22,75% in relation on the dry biomass. For light with a wavelength of 470 nm, the best result for biomass production was 1270 mg/L, while the best result of lipid contente was 19,03% in relation to the dry biomass
 
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EQD19002_C.pdf (3.43 Mbytes)
Publishing Date
2019-05-28
 
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