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Master's Dissertation
DOI
10.11606/D.97.2012.tde-27082013-105559
Document
Author
Full name
Rafael Rodrigues Philippini
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Lorena, 2012
Supervisor
Committee
Silva, Silvio Silverio da (President)
Carvalho, Walter de
Maugeri Filho, Francisco
Title in Portuguese
Variedades híbridas de bagaço de cana-de-açúcar: caracterização química e hidrólise enzimática em condições de pré-tratamento difrenciadas
Keywords in Portuguese
Bagaco de cana-de-acucar
Celulose
Hemicelulose
Hidrolise acida
Hidrolise enzimatica
Lignina
Abstract in Portuguese
O presente trabalho teve como objetivo, a caracterizacao da composicao quimica de diferentes variedades de bagaco de cana-de-acucar (CTC-9; CT99-1906; SP81-3250; RB86-7515 e CT99-1902), e posterior hidrolise enzimatica para averiguacao de acucares redutores e sacarificacao da biomassa. As analises composicionais foram realizadas utilizando biomassa nas condicoes in natura, celulignina e polpa celulosica. As condicoes de pre-tratamento das amostras para hidrolise com acido sulfurico diluido (relacao s/l: 1,5:10; temperatura: 150 °C; concentracao acida: H2SO4 2% m/v; tempo: 30 minutos); e deslignificacao com hidroxido de sodio (1:10 s/l; 100 °C; 1% NaOH; 30 minutos) foram pre-estabelecidas de modo a determinar uma analise comparativa das diferentes variedades de bagaco de cana-de-acucar estudadas. A hidrolise enzimatica das amostras obtidas apos os pre-tratamento foram realizadas em frascos Erlenmeyers de 125 mL, contendo tampao citrato (50 mM; pH 5; 1:20 s/l) e carga enzimatica (Celluclast 1.5 L - 75 FPU/mL e 13,5 UI/mL de ?-glicosidase; e Novozym 188 - 65 UI/mL de ?-glicosidase), e surfactante Tween 20 (0,15 g/g de bagaco). A composicao quimica das variedades de bagaco de cana-de-acucar, acucares redutores e sacarificacao da biomassa foram determinados por metodos cromatograficos, espectrofotometricos e gravimetricos tradicionais. Quanto a analise das variedades de cana tratada, a media dos valores observados: in natura - celulose (40,84%), hemicelulose (24,07%), lignina (33,7%) e cinzas (0,68%); extraido - celulose (38,83%), hemicelulose (27,32%), lignina (25,92), cinzas (0,32%) e extrativos (10,24%); celulignina - celulose (54,17%), hemicelulose (5,3%), lignina (37,28%), cinzas (0,54%) e polpa celulosica - celulose (77,48%), hemicelulose (6,07%), lignina (15,4%) e cinzas (0,32%). Os processos de hidrolise acida e deslignificacao demonstraram remocao eficaz da hemicelulose e da lignina (80% e 58%, respectivamente), e solubilizacao da biomassa (45% e 14%, em media). Os percentuais de sacarificacao da celulose das amostras apos 24 horas de reacao foram de 9,7% para bagaco extraido, 50,4% para celulignina e 72,87% para polpa celulosica. Nao foram observadas variacoes significativas na composicao quimica das cinco amostras estudadas, bem como diferenciacao na sacarificacao das mesmas. Evidenciou-se que emprego de variedades de bagaco de cana-de-acucar distintas utilizando pre-tratamentos em condicoes fixas nao alteram os percentuais quimico-estruturais do vegetal, bem como a recuperacao da glicose e dos acucares redutores presente na celulose.
Title in English
Hybrid sugarcane bagasse varieties: Chemical characterization and enzymatic hydrolysis in different pretreatment conditions
Keywords in English
Acid hydrolysis
Cellulose
Enzymatic hydrolysis
Hemicellulose
Lignin
Sugarcane bagasse
Abstract in English
The present work had as objective the chemical characterization of different varieties of sugarcane bagasse (CTC-9; CT99-1906; SP81-3250; RB86-7515 e CT99-1902) in different pretreatment conditions and the influence of pretreatments concerning varieties, reducing sugars recovery and biomass saccharification. Different pretreated biomass were obtained from in natura bagasse as it follows: extracted bagasse (24 hours/24 hours water/ethanol extraction in Sohxlet equipment), cellulignin (solid/liquid ratio: 1.5/10; temperature: 150 °C; acid concentration: H2SO4 2% w/v; residence time: 30 minutes); and cellulose pulp (1:10 solid/liquid ratio; 1% NaOH w/v; 100°C; 30 minutes). Enzymatic hydrolysis experiments were performed in 125 mL Erlenmeyers containing citrate buffer (50 mM; pH 5; 1:20 solid/liquid ratio), Tween 20 surfactant (0.15 g/g of bagasse) and commercial enzymatic loads of Celluclast 1.5 L (75 FPU/mL and 13,5 IU/mL of ?- glicosidase) and Novozym 188 (65 IU/mL of ?-glucosidase). Chemical composition of varieties, reducing sugars and biomass saccharification were determined by chromatographic, spectrometric and gravimetric traditional methods. Biomass presented an average composition in each condition as it follows: In natura - cellulose (40.84%), hemicellulose (24.07%), lignin (33.7%) and ashes (0.68%); Extracted - cellulose (38.83%), hemicellulose (27.32%), lignin (25.92), ashes (0.32%) and extractives (10.24%); Cellulignin - cellulose (54.17%), hemicellulose (5.3%), lignin (37.28%) and ashes (0.54%). Cellulose pulp - cellulose (77.48%), hemicellulose (6.07%), lignin (15.4%) and ashes (0.32%). Acid and alkaline hydrolysis presented effective removal of hemicellulose and lignin (80% and 58% respectively), showing as average biomass solubilization 45% and 14%. Saccharification after 24 hours of reaction presented 9,7% for extracted bagasse, 50.4% for cellulignin and 72.87% for cellulose pulp. There were no significant observations in chemical-structural composition and at cellulose saccharification of the five studied samples, showing that the variety did not present any relevant influence on pretreated sugarcane bagasse.
 
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Publishing Date
2013-08-27
 
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