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Master's Dissertation
DOI
10.11606/D.9.2018.tde-29012018-154046
Document
Author
Full name
Denize Duarte Pereira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Chasin, Alice Aparecida da Matta (President)
Carvalho, Valdemir Melechco
Pinto, Ernani
Title in Portuguese
Determinação de Flunitrazepam e 7¬aminoflunitrazepam em soro por cromatografia líquida de alta eficiência acoplada à espectrometria de massa em tandem com a utilização de extração on line: aspecto forense
Keywords in Portuguese
7-aminoflunitrazepam
Análise toxicológica
Drogas de abuso
Fármacos psicotrópicos (Uso)
Flunitrazepam
LC_MS/MS
Toxicologia Forense
Abstract in Portuguese
Atualmente os benzodiazepínicos constituem o grupo de fármacos de prescrição mais consumidas em todo o mundo. Além da utilização desta classe farmacológica como fármacos de abuso, mais recentemente seu uso está associado a uma nova cultura psicodélica emergente. O termo "club drugs" tem sido usado para descrever estes fármacos que levam a efeitos psicodélicos e euforizantes. Acresça-se a isto à preocupante utilização destas substâncias em situações de estupro e/ou assalto, as chamadas "drug-facilitated sexual assault", destacando-se o flunitrazepam. Devido às pequenas doses administradas e a extensiva metabolização, a identificação do flunitrazepam e seus metabólitos torna-se dificultada em relação a outros benzodiazepínicos. O presente trabalho constitui validação de metodologia analítica que permita a correta identificação e quantificação de flunitrazepam e seu principal produto de biotransformação, o 7-aminoflunitrazepam, em soro. O método desenvolvido mostrou boa linearidade, precisão, exatidão, rendimento e capacidade de detectar os analitos mesmo em baixas concentrações, permitindo desta forma a inferência sobre a realidade dos casos onde se utiliza este fármaco sem fins terapêuticos.
Title in English
Determination of flunitrazepam e 7-aminoflunitrazepam in serum by high performance liquid chromatography tandem mass spectrometry using on line extraction: "forensic aspect"
Keywords in English
7-aminoflunitrazepam
Abuse drugs
Flunitrazepam
Forensic toxicology
LC_MS / MS
Psychotropic drugs (Use)
Toxicological analysis
Abstract in English
Currently the benzodiazepines are one of the most consumed groups among the prescripton drugs in the world. Beside this, they have been used as drugs of abuse and more recently their use is associated with the new emerging psychodelic culture. The term "club drugs" has been used to describe these drugs that cause psychodelic and euphoric effects. In addition to this, there is a growing concern with the use of these substances related to "drug-facilitated sexual assault", among of which Flunitrazepan is pointed out as one of the most important . Due to the small doses involved in the consumption for this purpose and also the extense biotransformation, the identification of this analyte and its metabolites become more difficult than that of other benzodiazeines. This study provides an analytical validation method that enables correct identification and quantification of Flunitrazepam and its main biotransformation product, the 7-aminoflunitrazepam, in serum. The method developed demonstrates good linearity, precision, accuracy, recovery and capacity of detect the analytes, even in low concentration. Thus making possible to makes inferences regarding the reality of cases where it is used as a drug without therapeutic use.
 
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Publishing Date
2018-01-29
 
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