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Master's Dissertation
DOI
10.11606/D.9.2004.tde-18022009-141456
Document
Author
Full name
Nádia Tawil
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Silva, Ovandir Alves (President)
Corrêa, Cristiana Leslie
Malbergier, André
Title in Portuguese
Determinação de etanol em saliva através do sistema enzimático Q.E.D.® e da cromatografia em fase gasosa
Keywords in Portuguese
Álcool
Análise toxicológica
Ar expirado
Etanol
Saliva (Análise)
Toxicologia forense
Toxicologia social
Abstract in Portuguese
O uso nocivo do álcool acarreta problemas de saúde para o usuário e prejuízos na sociedade. Vários espécimes biológicos podem ser utilizados para verificar a exposição a bebidas alcoólicas. No trabalho foi investigada a utilização da saliva na determinação de etanol através das técnicas: enzimática Q.E.D. A-150 de triagem - e cromatografia em fase gasosa, após separação por aquecimento, Headspace de confirmação. O método foi padronizado, validado e aplicado em amostras de saliva provenientes de voluntários que ingeriram de maneira controlada bebida alcoólica. Em todas as amostras analisadas foi possível detectar a concentração de etanol, observando a existência de uma diferença entre os respectivos resultados das duas técnicas. Os resultados interindividuais apresentaram grande variabilidade nos mesmos tempos de coleta, apesar da ingesta equivalente de etanol. No estudo foi verificado que a saliva é uma matriz alternativa adequada para verificar a concentração de etanol em indivíduos expostos às bebidas alcoólicas.
Title in English
Analysis of ethanol in saliva through enzymatic Q.E.D.® - screening test, and of headspace gas chromatography.
Keywords in English
Alcohol
Breath
Ethanol
Forensic Toxicologycal
Saliva (Analysis)
Social Toxicologycal
Toxicologycal analysis
Abstract in English
The abuse of alcohol leads to health injury and causes damage to the society. Several biological matrices can be used to monitor exposure to alcoholic beverages. In this work, saliva was used in the determination of ethanol for both techniques: enzymatic Q.E.D. A-150 screening test -, and headspace-gas chromatography - confirmation test -. The method was developed, validated and used for the analysis of saliva samples from volunteers who ingested alcoholic beverages in a controlled schedule. Ethanol was detected and in all samples, noticing a difference between the results of both techniques. Inter-volunteers results showed high variability for the same collection times, although the amount of ethanol ingested was equivalent. These results suggest that saliva is a good option for the in field determination of ethanol in exposed individuals to alcoholic beverages.
 
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Publishing Date
2013-06-21
 
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