O objetivo desta pesquisa foi o desenvolvimento e validação de métodos analíticos quantitativos para a determinação de fluoroquinolonas de 2ª e 3ª gerações. Um método espectrofotométrico e um método cromatográfico, simples e rápido foi desenvolvido e validado para a determinação quantitativa de seis fármacos quinolônico e doze preparações farmacêuticas, entre comprimidos e soluções injetáveis. As fluoroquinolonas estudadas foram ciprofloxacino (CIP), gatifloxacino (GAT), levofloxacino (LEV), lomefloxacino (LOM), norfloxacino (NOR) e pefloxacino (PEF). As técnicas empregadas para a caracterização dos padrões secundários foram a análise elementar, análise térmica (TG/DTG) e DSC e espectroscopia no infravermelho. As quinolonas foram analisadas por espectrofotometria direta no UV empregando metanol como solvente. Na cromatografia líquida, as condições padronizadas foram: coluna LiChrospher® 100 RP-18 (5µm, 125 x 4 mm), e fase móvel constituída de água:acetonitrila (80:20 v/v), com 0,35% de trietilamina, pH 3,3 ajustado com ácido fosfórico. A vazão empregada foi de 1,0 mL/min e a análise foi desenvolvida usando detecção UV variando de 288 a 295 nm, em temperatura de 24 ± 2 °C. todas as quinolonas foram separadas em menos de 5 min. As curvas de calibração foram lineares (r > 0,9999. ) no intervalo de concentração de 4,0 a 24,0µg/mL. O desvio padrão relativo (DPR) foi < 1,0% e a média de recuperação dos comprimidos foi acima de 98,0%. Os dados obtidos indicam precisão e exatidão dos métodos propostos.

The objective of this research was to develop and validate analytical methods for quantitative determination of fluoroquinolones of second and third generation. A simple and rapid spectrophotometric and chromatographic method was developed and validated for quantitative determination of six quinolone antibiotics in twelve pharmaceutical preparations, amongst them tablets and injection preparations. The fluoroquinolones selected in this research were ciprofloxacin (CIP), gatifloxacin (GAT), levofloxacin (LEV), lomefloxacin (LOM), norfloxacin (NOR) and pefloxacin (PEF). Elemental analysis, thermal analysis (TG/DTG) e DSC and infrared spectrophotometric techniques were used to characterize and qualify raw material and were used as secondary standards. The quinolones were quantitatively determined using direct UV spectrophotometry with methanol as solvent. The liquid chromatographic method was constituted of a LiChrospher® 100 RP-18 column (5µm, 125 x 4 mm) and a mobile phase constituted of water:acetonitrile (80:20 v/v), with 0.3% of triethylamine and pH adjusted to 3.3 with phosphoric acid. The flow rate was 1.0 mL/min and the analyses were performed using UV detector with wavelengths varying from 288 to 295 nm. All analyses were performed at room temperature (24 ± 2 °C) and were separated within 5 min. The calibration curves were linear for both, spectrophotometric and chromatographic methods with correlation coefficient better than 0.9999. The average recovery values were above 98.0% with relative standard deviation (RSD) < 1.0%. The obtained data indicates accuracy and precision of the proposed methods.