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Doctoral Thesis
DOI
10.11606/T.9.2002.tde-22052015-172120
Document
Author
Full name
Claudio Picanço Magalhães
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2002
Supervisor
Committee
Finardi Filho, Flavio (President)
Marquez, Ursula Maria Lanfer
Pueyo, Manuel Troyano
Rodriguez, Maria Ines Genovese
Sá, Maria Fátima Grossi de
Title in Portuguese
Análise molecular da interação entre a α-amilases de Z.subfasciatus e o inibidor de α-amilases de trigo 0.19: aspectos biotecnológicos para o controle de pragas de armazenamento
Keywords in Portuguese
Alimentos de origem vegetal (Armazenagem)
Antinutricional
Armazenagem de alimentos
Trigo
Wheat
Zabrotes subfaciatus
Abstract in Portuguese
Sementes de feijão são freqüentemente infestadas por diferentes insetos-praga incluindo Zabrotes subfasciatus, Callosobruchus maculatus e Acanthocelides obtectus. Estratégias visando o controle do ataque desses insetos têm sido desenvolvidas através do estudo de substâncias tóxicas e/ou proteínas antinutricionais que atuem sobre o desenvolvimento destes insetos. Os inibidores protéicos de α-amilases presentes em leguminosas como o feijão comum, bem como em sementes de cereais como o trigo, têm a capacidade de inibir a α-amilase de Z. subfasciatus. O estudo do mecanismo de interação entre os inibidores e as enzimas digestivas de insetos pode propiciar o delineamento de inibidores potentes que atuariam especificamente contra essas pragas. O estudo da interação do inibidor de trigo 0.19 e com a α-amilase de Z. subfasciatus desenvolvido neste trabalho demonstra a atuação de vários componentes de força na superfície de interação entre a enzima e o inibidor, garantindo a estabilidade do complexo. A presença de pontes de hidrogênio, interações hidrofóbicas e ligações iônicas foram evidenciadas. Através da análise da seqüência primaria de outros inibidores de cereais, foi verificado que o inibidor de α-amilase de trigo denominado 0.53 e o inibidor de cevada possivelmente atuam da mesma maneira sobre a α- amilase do inseto. Estes resultados tornam esses inibidores protéicos potenciais candidatos à transformação de plantas de feijão visando o controle do desenvolvimento de Z. subfasciatus durante seu estágio larval.
Title in English
Molecular analysis of the interaction between α-amylases Z.subfasciatus and wheat α-amylases inhibitors 0.19: biotechnological aspects for controlling storage pests
Keywords in English
Anti-nutritional
Plant foods (Storage)
Storage of food
Wheat
Zabrotes subfaciatus
Abstract in English
Several insects included Callosobruchus maculatus, Acanthocelides obtectus and Zabrotes subfasciatus have frequently infest bean seeds. Strategies for the control of these predators have been developed through the study of toxic substances and antinutritional factors, which act on the development of these insects. Proteinaceus Inhibitors of α-amylases present in varieties of beans, as well as in wheat seeds have been demonstrated to be active to the α-amylase of Z. subfasciatus. The study of the mechanism of interaction between these inhibitors and the digestive enzymes can promote a powerful inhibitor that acts specifically against these insect-pest. Studies on the interaction of the inhibitor of wheat kernels 0.19 and Z. subfasciatus α-amylase demonstrated the performance of some intermolecular forces on the surface of the enzyme-inhibitor interaction giving the stability to the complex. The presence of hydrogen bond, hydrophobic interactions and saline bridges had been showed . The analysis of the primary sequences of inhibitors from other cereals allowed to verify that the wheat amylase inhibitor 0.53 and the barley inhibitor possibly act by the same way on the insect a-amylase. These results make these proteinaceus inhibitors potential candidates to be used on the plant transformation for the control of the development of Z. subfasciatus during of larval stage.
 
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Publishing Date
2015-05-22
 
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