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Doctoral Thesis
DOI
https://doi.org/10.11606/T.9.2003.tde-22022022-123624
Document
Author
Full name
Jacqueline Dettman Bierhals
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2003
Supervisor
Committee
Cordenunsi, Beatriz Rosana (President)
Buckeridge, Marcos Silveira
Kluge, Ricardo Alfredo
Lajolo, Franco Maria
Rombaldi, Cesar Valmor
Title in Portuguese
Enzima desramificadora de amido tipo-isoamilase durante o amadurecimento de bananas (Musa acuminata AAA, Grupo Cavendish, cv. Nanicão)
Keywords in Portuguese
Amido (Metabolismo)
Banana (Análise)
Bioquímica de alimentos
Enzimas
Sacarose (Metabolismo)
Abstract in Portuguese
As enzimas desramificadoras (DBE) são indispensáveis para a hidrólise das ligações α(1-6) do amido. Uma DBE tipo-isoamilase foi detectada durante o amadurecimento de banana, apresentando maior afinidade por β-dextrina limite, seguida por glicogênio, com tamanho de subunidade estimado em 80 kDa. O seqüenciamento de parte do gene da enzima de banana mostrou alta homologia com isoamilase de outros vegetais, confirmando sua identidade. A isoamilase de banana apresentou atividade, transcrição e expressão constantes durante o amadurecimento. Quando amostras de banana foram tratadas com 10 ppm de etileno, foi observada uma diminuição no nível de transcrito de isoamilase, porém sem alterações no perfil de atividade e quantidade de proteína expressa, sugerindo que a isoamilase de banana não é regulada por etileno.
Title in English
Enzima desramificadora de amido tipo-isoamilase durante o amadurecimento de bananas (Musa acuminata AAA, Grupo Cavendish, cv. Nanicão)
Keywords in English
Amido (Metabolismo)
Banana (Análise)
Bioquímica de alimentos
Enzimas
Sacarose (Metabolismo)
Abstract in English
Debranching enzyme (DBE) are indispensable to break α(1-6) bounds of starch. One DBE type-isoamylase was detected during banana ripening, which showed higher affinity for β-dextrin limit, followed for glycogen, with subunit size of 80 kDa. The sequencing of the part gene of the enzyme showed high homology with isoamylase of others vegetables, confirming its identify. Isoamylase of banana showed constant activity, transcription and expression during ripening. When banana samples were treated with 10 ppm of ethylene was observed a decrease in transcript level, but without changes in activity and amount protein profile, sugaring that isoamylase of banana is not regulated for ethylene.
 
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Publishing Date
2022-02-22
 
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