Disertación de Maestría
DOI
https://doi.org/10.11606/D.9.1982.tde-01112012-105344
Documento
Autor
Nombre completo
Adelaide Tie Catutani
Dirección Electrónica
Instituto/Escuela/Facultad
Área de Conocimiento
Fecha de Defensa
Publicación
São Paulo, 1982
Director
Título en portugués
Pectinesterase do mamão (Carica papaya L.)
Palabras clave en portugués
Ativação enzimática (Pesquisa - Características)
Bioquímica de alimentos
Carica papaya
Mamão
Pectinesterase
Propriedades bioquímicas
Proteínas (Estudo - Pesquisa)
Purificação enzimática
Resumen en portugués
Não consta resumo na publicação.
Título en inglés
Pectinesterase from papaya (Carica papaya L.)
Palabras clave en inglés
Biochemical properties
Carica papaya
Enzimatic activation
Enzimatic purification
Food biochemistry
Pectinesterase
Proteins
Resumen en inglés
Pectinesterase (E C 3.1.1.11) was extracted from papaya (Carica papaya L.) tissue and purified 4,48 fold by fractionated ammonium sulphate precipitation, dialysis and chromatography on DEAE-celulose and Sephadex G-100. Extraction conditions of enzyme were studied and their properties characterized. The increase on the activity of pectinesterase was practically followed by increase on the content of soluble pectin, during ripening. The molecular weight of the enzyme eluted in one peak of activity was 53.000 daltons. The pectinesterase has its maximum activity at pH 8,0 and at 0,2 M of NaCl. Optimum temperature for the enzyme assay was 60°C. The enzymatic reaction was linear with the time and protein concentration. With citric pectin as substrate, pectinesterase had a Km of 0,012% and was inhibited competitively by polygalacturonic acid with a Ki of 0,007%. Papaya pectinesterase was inhibited by sucrose, glucose and glicerol.
ADVERTENCIA - La consulta de este documento queda condicionada a la aceptación de las siguientes condiciones de uso:
Este documento es únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro. Esta reserva de derechos afecta tanto los datos del documento como a sus contenidos. En la utilización o cita de partes del documento es obligado indicar el nombre de la persona autora.
Fecha de Publicación
2012-11-01