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Master's Dissertation
DOI
10.11606/D.88.2007.tde-01122014-112608
Document
Author
Full name
Elys Raquel Andrade Ferreira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2007
Supervisor
Committee
Goncalves, Debora (President)
Guimaraes, Francisco Eduardo Gontijo
Mascaro, Lucia Helena
Title in Portuguese
Filmes de polipirrol como matrizes para a imobilização da polifenol oxidase e aplicação como biossensores amperométricos na análise de compostos fenólicos
Keywords in Portuguese
Biosensores
Polifenol oxidase (PFO)
Polipirrol (PPI)
Abstract in Portuguese
Nesta dissertação, a polifenol oxidase (PFO) como extrato bruto de abacate (Persea americana) foi imobilizada em filmes de polipirrol (PPI) sintetizados eletroquimicamente utilizando o glutaraldeído (GA) como um agente de ligação entrecruzada. Os filmes PPI e PPI/PFO-GA foram caracterizados por eletroquímica, principalmente voltametria cíclica, sendo avaliadas a eletroatividade e a reversibilidade eletroquímica. A detecção de compostos fenólicos em soluções padrão foi feita por cronoamperometria, tendo um controle sobre a concentração dos compostos. O processo de transferência de massa foi monitorado com uma microbalança de cristal de quartzo eletroquímica. Os resultados indicaram uma boa reprodutibilidade das medidas na detecção dos compostos fenólicos. A estabilidade do biossensor em uma solução tampão manteve-se durante 27 dias, um resultado aceitável já que é encontrado na literatura um tempo de vida estável para sistemas semelhantes em tomo de 30 dias
Title in English
Polypyrrole film as matrix for the immobilization of polyphenol oxidase and application as amperometric biosensors in the analysis of phenolic
Keywords in English
Biosensors
Polyphenol oxidase (PPO)
Polypyrrole (PPY)
Abstract in English
In this dissertation, polyphenol oxidase (PPO) as crude extract of avocado (Persea americana) was immobilized on electrochemical1y synthesized polypyrrole (PPY) films using glutaraldehyde (GA) as a crosslinking agent. PPY and PPY/PPO-GA films were electrochemical1y characterized, mainly by cyclic voltametry, where electroactivity and electrochemical reversibility were evaluated. The detection of phenolic compounds in standard samples was made by chronoamperometry with a control over the compound concentration. The process of mass transfer was monitored with an electrochemical quartz crystal microbalance (EQCM). Our results indicated a good repeatability of the measurements for the detection of phenolic compounds. The stability of biosensor in a buffer solution has remained for 27 days, a result acceptable since it is found in the literature a time of life stable for similar systems around 30 days
 
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Publishing Date
2014-12-03
 
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