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Doctoral Thesis
DOI
10.11606/T.87.2009.tde-30042010-090819
Document
Author
Full name
Flávia Mandolesi Pereira de Melo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Melo, Itamar Soares de (President)
Araujo, Janete Magali de
Oliveira, Valéria Maia de
Souza, Ana Olívia de
Vicente, Elisabete Jose
Title in Portuguese
Bioprospecção de Actinobactérias da rizosfera de milho ( Zea mays L.) com atividade antifúngica
Keywords in Portuguese
Actinobactérias rizosféricas
Bioprospecção
Controle Biológico
Cromatografia
Metabólitos secundários
PKS
Abstract in Portuguese
Actinobactérias ocorrem em diversos ambientes e possuem grande potencial na produção de enzimas, antibióticos e fármacos. O presente trabalho teve como objetivo o isolamento, a identificação e a bioprospecção de actinobactérias da rizosfera de milho. Foram isoladas 60 linhagens de actinobactérias de plantas sadias de milho, cultivadas em diferentes regiões do Estado de São Paulo. Os isolados com maior atividade, CCMA30 e CCMA33, foram identificados por meio do seqüenciamento parcial do gene 16S rRNA como pertencentes à espécie Streptomyces griseorubiginosus, nestes isolados foi também detectada a presença de genes PKS.O fracionamento do extrato bruto da linhagem CCMA 33 revelou que a fração 7 (m/z 719-768) com atividade antifúngica, foi submetida a fragmentação (MS/MS), onde pelas buscas no dicionário de produtos naturais confirmou-se a presença de Julichromes Q 6-6, substância sintetizada por policetídeos e de mais duas Julichromes (Q 1-3 e Q 3-3). Estes resultados evidenciam a produção de biocompostos de interesse biotecnológico por actinobactérias da rizosfera.
Title in English
Bioprospection of actinobacteria from maize (Zea mays L.) rhizosphere with antifungal activity.
Keywords in English
Biological control
Bioprospection
Chromatograph
PKS
Rhizosphere actinobacteria
Secondary metabolites
Abstract in English
Actinobacteria occur in different environments and have a great potential in producing enzymes, antibiotics and medical molecules. The present work aimed the isolation, identification and the bioprospection by rhizosphere actinobactérias. Sixty strains were isolated from healthy plants of maize, cultivated in different regions Socorro, Serra Negra and Ribeirão Preto. The two isolates with higher activity CCMA30 and CCMA33 were identified by the partial sequencing of the 16S rDNA gene as belonging to the species Streptomyces griseorubiginosus. In these isolates it was detected the presence of PKS genes. The fractioning of the crude extract of the strain CCMA 33 has revealed that fraction 7 (m/z 719-768) displayed antifungal activity and it was submitted to fragmentation (MS/MS), where by searches in the dictionary of natural products detected the presence of Julichromes Q6-6 and the presence of two other Julichromes (Q1-3 and Q3-3). These results highlight the production of secondary metabolites of biotechnological interest by rhizosphere actinobacteria.
 
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Publishing Date
2010-05-26
 
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