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Doctoral Thesis
DOI
https://doi.org/10.11606/T.87.2007.tde-26012012-170618
Document
Author
Full name
Patrícia Locosque Ramos
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Moreira Filho, Carlos Alberto (President)
Brocchi, Marcelo
Marques, Marilis do Valle
Pasternak, Jacyr
Schenberg, Ana Clara Guerrini
Title in Portuguese
Taxonomia do gênero Stenotrophomonas através de Multi Locus Sequence Analysis (MLSA).
Keywords in Portuguese
Bactérias gram-negativas
Fixação de nitrogênio
Genética bacteriana
Reação em cadeia por polimerase
Abstract in Portuguese
As Stenotrophomonas são comumente encontradas no trato respiratório de pacientes com doenças pulmonares crônicas e também na rizosfera de plantas. Esse gênero apresenta resistência a diversos antibióticos, promove o crescimento de plantas e algumas espécies apresentam a capacidade de fixar o nitrogênio atmosférico. O Multi Locus Sequence Analysis (MLSA) é uma metodologia baseada em genes constitutivos para definição e alocação taxonômica de novas espécies. O objetivo geral do presente trabalho foi caracterizar taxonomicamente uma coleção ampla de Stenotrophomonas composta por isolados endófitos, linhagens-tipo e de referência. Para tanto, foi estabelecido um sistema de classificação e identificação de Stenotrophomonas por meio de MLSA. Foi possível através da metodologia de MLSA definir 9 novas espécies, detectar a presença de um novo gênero e estabelecer um sistema online de taxonomia para Stenotrophomonas.
Title in English
Taxonomy of Stenotrophomonas genus by means of Multi Locus Sequence Analysis (MLSA).
Keywords in English
Bacterial genetics
Gram-negative bacteria
Nitrogen fixation
Polymerase chain reaction
Abstract in English
The genus Stenotrophomonas is found in the respiratory treatment of patients with chronic pulmonary and also in the rizhosfera of plants. It presents resistance to several antibiotics, promotes the growth of plants and some species present the ability to fix atmospheric nitrogen. The Multi Locus Sequence Analysis (MLSA) is a methodology based on constitutive genes for definition and taxonomic allocation of new species. The general objective of the present work was to characterize a wide collection constituted by Stenotrophomonas from isolated endophytic, type and reference strains. In such a way, a system of classification and identification of Stenotrophomonas by means of MLSA was established. It was possible through the MLSA methodology to define 9 new species, to detect the presence of a new genus and to establish an online system for Stenotrophomonas taxonomy.
 
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Publishing Date
2012-02-07
 
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