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Master's Dissertation
DOI
10.11606/D.87.2017.tde-23032017-151831
Document
Author
Full name
Jupciana Martins Figueredo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Nascimento, Ana Lucia Tabet Oller do (President)
Guth, Beatriz Ernestina Cabilio
Vicente, Elisabete Jose
Title in Portuguese
Avaliação do papel de duas proteínas de Leptospira interrogans na patogênese da leptospirose.
Keywords in Portuguese
Leptospira
Leptospirose
Proteínas recombinantes
Abstract in Portuguese
Leptospirose é uma zoonose mundial que acomete várias espécies de mamíferos, incluindo humanos, causada por espécies de bactérias patogênicas do gênero Leptospira. Possui um quadro de manifestação clínico muito variado, podendo apresentar desde sintomas comuns a outras doenças como febre, calafrios, cefaleia, dores musculares, enjoo, vômitos, diarreia e uma forma mais severa da infecção denominada síndrome de Weill. Seguindo a estratégia de genômica funcional, foram selecionados genes de Leptospira interrogans sorovar Copenhageni, LIC10377, LIC11122, LIC11184 e LIC12287, tendo como critério a predição de localização das proteínas na membrana. Os fragmentos referentes aos genes LIC11122 e LIC12287 foram clonados no vetor pGEM-T Easy e subclonados no vetor de expressão pAE. As proteínas avaliadas interagem com laminina e plasminogênio, de forma dose-dependentes e saturáveis, sugerindo atuam nos processos de patogênese da bactéria. A presença de um fator sigma na superfície celular desempenhando um papel secundário, sugere que a rLIC11122 pode ser uma proteína moonlight.
Title in English
Role of two Leptospira interrogans lipoproteins in the pathogenesis of leptospirosis.
Keywords in English
Leptospira
Leptospirosis
Recombinant proteins
Abstract in English
Leptospirosis is a worldwide zoonosis that affects several species of mammals, including humans, caused by species of pathogenic bacteria of the genus Leptospira. It has a very varied clinical manifestation board and may have symptoms from common tropical diseases such as fever, chills, headache, muscle aches, nausea, vomiting, diarrhea and a severe syndrome of infection known as Weill syndrome. Following the functional genomics strategy, genes were selected from Leptospira interrogans serovar Copenhageni, LIC10377, LIC11122, LIC11184 and LIC12287, with the criterion of the prediction location of proteins in the membrane. The fragments related to genes LIC11122 and LIC12287 were cloned into pGEM-T Easy vector and subcloned into the expression vector pAE. The evaluated proteins interact with laminin and plasminogen, dose-dependent and saturable manner, suggesting participation in bacterial pathogenesis processes. The presence of a sigma factor on the cell surface plays a secondary role, suggests that performs a moonlight rLIC11122 protein.
 
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Publishing Date
2017-03-23
 
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