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Doctoral Thesis
DOI
https://doi.org/10.11606/T.87.2020.tde-15092023-091511
Document
Author
Full name
Henriette Righi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2020
Supervisor
Committee
Arruda Neto, Joao Dias de Toledo (President)
Hormaza, Joel Mesa
Menck, Carlos Frederico Martins
Monteiro, Gisele
Silva, Evandro Drigo da
Silva, Luiziana Ferreira da
Title in Portuguese
Estudo de efeitos biofísicos induzidos pela combinação de exposição à Radiação Ionizante e a Campos Magnéticos
Keywords in Portuguese
Deinococcus radiodurans
Campo Magnético Estático
Efeitos biofísicos
Radiação Ionizante
Recuperação celular
Abstract in Portuguese
Campos Magnéticos de moderadas intensidades eficientemente provocam alterações em sistemas biológicos. Resultados obtidos neste Laboratório com células expostas a gamas e a Campos Elétricos Estáticos (CEE), indicaram ser este um eficiente radiossensitizador. Um CEE de 200V provocou drástica redução no ombro de reparo da D. radiodurans. Questões não respondidas, referentes à ação de Campos Magnéticos Estáticos (CME): (1) indução de efeitos biofísicos em células íntegras, e (2) interferência na viabilidade de células irradiadas. D. radiodurans, a espécie mais resistente à radiação ionizante, foi irradiada com gamas entre 0.5 e 13 kGy, no Instituto de Pesquisas Nucleares (IPEN), e imediatamente submetida aos CMEs de 0,08 T e 0,8 T. E. coli foi utilizada como controle negativo. Principais conclusões: (a) a exposição de células de D. radiodurans a altas doses de radiação e em seguida a CMEs, aumenta sua viabilidade; (b) a recuperação das células irradiadas é proporcional à intensidade do CME; (c) o processo de recuperação celular (aumento da viabilidade) é estocástico.
Title in English
Study of biophysical effects induced by the combination of exposure to Ionizing Radiation and Magnetic Fields
Keywords in English
Deinococcus radiodurans
Biophysical effects
Cell recuperation
Ionizing Radiation
Static Magnetic Fields
Abstract in English
Moderate intensities Magnetic Fields induce changes in biologic systems. Results from this Laboratory with cells exposed to both gammas and Static Electric Fields (SEF), indicates this agent as an efficient radiosensitizer. A 200V SEF caused accentuated depletion of D. radiodurans repair shoulder. Unanswered questions related to the role of Static Magnetic Fields (SMF) are: (1) induction of biophysical effects in whole cells, and (2) interference effects in irradiated cells viability. D. radiodurans, a resistant species to ionizing radiation, was exposed to gammas between 0.5 and 13 kGy, at the Nuclear Research Institute (IPEN), and immediately after exposed to SMFs of 0.08 T and 0.8 T. E. coli was employed as negative control. Mainly conclusions are: (a) exposure of D. radiodurans cells to both high doses of radiation and SMF increases its viability; (b) the viability recuperation of irradiated cells is proportional to the SMF intensity; (c) the cell recuperation process (viability increase) is stochastic.
 
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Publishing Date
2023-09-19
 
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