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Doctoral Thesis
DOI
https://doi.org/10.11606/T.87.2019.tde-12092023-112731
Document
Author
Full name
Augusto Cesar Crivellari
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2019
Supervisor
Committee
Sluys, Marie Anne van (President)
Alcalde, Felipe Santiago Chambergo
Buckeridge, Marcos Silveira
Domingues, Douglas Silva
Souza, Robson Francisco de
Title in Portuguese
Prospecção por Bioinformática de pequenos RNAs na relação de fungos fitopatogênicos e seus hospedeiros
Keywords in Portuguese
Fungos fitopatogênicos
pequenos RNAs não-codificantes
Retrotransposons
Abstract in Portuguese
Pesquisadores demonstraram que o fungos podem produzir produz pequenos RNAs papel relevante no processo de estabelecimento de uma infecção em seu hospedeiro, suprimindo a expressão de proteínas relevantes ao hospedeiro, sugerindo que não somente proteínas poderiam agir como efetoras no estabelecimento da infecção. O presente trabalho prospectou em 27 genomas de fungos patogênicos de relevância comercial, retrotranposons que serviram de base para passos de busca, por análise de semelhança, de possíveis pequenos RNAs efetores. Também foi averiguado nos genomas de fungos escolhidos a presença de enzima relevantes no processamento de pequenos RNAs como a Argonauta e Dicer. Ficou claro que não há aparente relação na presença e distribuição das enzimas do maquinário de processamento de mirRNAs e as estratégias ecológicas de cada fungos. A análise de semelhança utilizada no alinhamento dos retrotransposons encontrados nos genomas dos fungos e os genomas das plantas hospedeiras de cada fungo, foi capaz de encontrar 210 sequências distintas de pequenos RNAs, os quais apresentaram 109 ocorrências de alinhamentos contra genes relacionado à defesa vegetal. Estas sequências encontradas, a presença de proteínas associadas ao processamento de pequenos RNAs nos fungos estudados e o locus de inserção destes pequenos RNAs no retrotransposons estudados, indicam que a estratégia deste trabalho foi bem- sucedida em encontrar potencias pequenos RNAs, que poderiam apresentar uma função interespecífica. Entretanto, este trabalho se beneficiaria da ampliação do universo amostral para confirmação estatística dos dados apresentados.
Title in English
smallRNA bioinformatics to surveil the relationship between pathogenic fungi and its hosts.
Keywords in English
Fitopathogenic Fungi
Retrotransposons
small non-coding RNAs
Abstract in English
Researchers have demonstrated that fungi can produce small RNAs that are relevant in the process of establishing an infection in their host, suppressing the expression of relevant proteins to the host, suggesting that not only proteins could act as effectors in the establishment of an infection. The present work prospected in 27 genomes of pathogenic fungi of commercial relevance, retrotransposons that served as a basis for search steps, by analysis of similarity, of possible small effector RNAs. It was also verified in the selected fungi genomes the presence of enzymes relevant in the processing of small RNAs such as Argonauta and Dicer. It was clear that there is no apparent relationship in the presence and distribution of the miRNA processing machinery enzymes and the ecological strategies of each fungi. The analysis of similarity used in the alignment of the retrotransposons found in the genomes of the fungi and the genomes of the host plants of each fungus, was able to find 210 distinct sequences of small RNAs, which presented 109 loci related to plant immunity. These sequences, the presence of proteins associated to the processing of small RNAs in the fungi studied and the locus of insertion of these small RNAs in the studied retrotransposons, indicate that the strategy of this work was successful in finding small RNAs, which could present an interspecific function. However, an expanded data set could provide stastistical confidence to the data.
 
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Release Date
2025-09-12
Publishing Date
2023-09-15
 
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