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Doctoral Thesis
DOI
10.11606/T.87.2014.tde-07052014-090937
Document
Author
Full name
Nara Diniz Soares Pessoa
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Stocco, Rita de Cassia (President)
Armelin, Hugo Aguirre
Kfoury Junior, José Roberto
Mendonça, Ronaldo Zucatelli
Santelli, Glaucia Maria Machado
Title in Portuguese
Estudos sobre a expressão do Papilomavírus Humano (HPV): avaliação comparativa sobre lesões cervicais, sangue periférico e retinoblastomas.
Keywords in Portuguese
Carcinoma
DNA viral
Genomas
HPV
Imunohistoquímica
Abstract in Portuguese
O HPV tem sido relacionado a diversos tipos de câncer, inclusive o cervical e o retinoblastoma. Este trabalho visa estudar a atividade viral pelo perfil clastogênico e expressão protéica em mulheres e crianças atendidas em Pernambuco. Amostras cervicais, de sangue periférico e retinoblastomas foram analisadas, sendo trinta e quatro mulheres e 7 crianças avaliadas. A detecção viral foi realizada por PCR e o HPV foi detectado em 29 mulheres (85,3%), com o tipo HPV-16 mais frequente. Através da imunoistoquímica e hibridização foi possível detectar o HPV em 6 crianças e as proteínas E1+E4, L1 e E6 foram evidenciadas nos tecidos em diversas regiões do olho, sugerindo atividade viral na amostra. Além disso, danos no material genético de mulheres com HPV foram envidenciados pelo ensaio cometa, sendo encontrada uma diferença estatística significante entre linfócitos em cultura de pacientes HPV-positivas comparadas àquelas HPV-negativas. Os dados trazem questionamentos sobre a presença de HPVs oncogênicos em tumores infantis e sua possível transmissão e mais evidências sobre os danos observados no DNA dos hospedeiros como consequência da presença e atividade virais.
Title in English
Studies on the expression of the Human Papillomavirus (HPV): comparative assessment in cervical lesions, peripheral blood and retinoblastomas.
Keywords in English
Carcinoma
Genomes
HPV
Immunohistochemistry
Viral DNA
Abstract in English
HPV has been linked to several cancers, including cervical and retinoblastoma. This work aims at to verify the viral activity by clastogenic profile and protein expression in women and children served in Pernambuco. Cervical samples, retinoblastomas and peripheral blood were analyzed, being thirty-four women and 7 children evaluated. Viral detection was performed by PCR and HPV was detected in 29 women, being the most frequent HPV-16. Through hybridization and immunohistochemistry analysis was possible to detect HPV in 6 children and E1+E4, L1 and E6 proteins were highlighted in the tissues in various parts of the eye, suggesting viral activity in the sample. In addition, damage to the genetic material of women with HPV have been showed by the Comet assay and found a significant statistical difference between lymphocytes in culture of HPV-positive patients compared to those HPV-negative. The data bring questions about the presence of oncogenic HPV in tumors and its possible transmission and more evidence about the damage observed in the DNA of the host as a result of the presence and viral activity.
 
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Publishing Date
2014-05-07
 
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