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Master's Dissertation
DOI
10.11606/D.87.2014.tde-06122014-085815
Document
Author
Full name
Demétria Luci Fazolo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Aniz, Patricia Antonia Estima Abreu de (President)
Azevedo, Anita Mitico Tanaka
Brandão, Paulo Eduardo
Title in Portuguese
Estudo da interação de prováveis lipoproteínas de membrana externa de Leptospira com proteínas do hospedeiro.
Keywords in Portuguese
Leptospira
Adesinas
Leptospirose
Lipoproteínas
Abstract in Portuguese
A leptospirose é uma zoonose mundial causada por espiroquetas patogênicas do gênero Leptospira, que colonizam os túbulos renais de animais domésticos e silvestres e são liberadas ao ambiente externo pela urina. Neste estudo avaliou-se a interação de seis prováveis lipoproteínas de membrana externa de leptospira com as proteínas do hospedeiro: colágeno I, colágeno IV, elastina, fibrinogênio, fibronectina celular e plasmática, laminina e plasminogênio. Os experimentos de adesão demonstraram que as proteínas recombinantes Lp21, Lp22 e Lsa30 apresentaram interação com os componentes do hospedeiro de maneira dose-dependente. Estas aderiram à fibronectina plasmática e laminina, além destes, a Lp21 e a Lp22 interagiram com plasminogênio, a Lp22 e a Lsa30 interagiram com colágeno IV. A Lp22 aderiu à elastina e ao fibrinogênio. No estudo de conservação gênica, os genes que codificam estas proteínas foram observados somente nas Leptospiras patogênicas. Portanto estas proteínas devem contribuir na adesão aos tecidos do hospedeiro na patogênese da Leptospira.
Title in English
Study of the interaction of probable outer membrane lipoprotein of Leptospira with host proteins.
Keywords in English
Leptospira
Adhesins
Leptospirosis
Lipoproteins
Abstract in English
Leptospirosis is a worldwide zoonosis caused by pathogenic spirochetes of the genus Leptospira that colonize the renal tubules of wild and domestic animals and are excreted in the environment by their urine. The aim of this work was to study the interaction of six leptospiral probable outer-membrane lipoproteins with host proteins: collagen I, collagen IV, elastin, fibrinogen, cellular fibronectin, plasma fibronectin, laminin, and plasminogen. The binding experiments demonstrated that the recombinant proteins showed interaction with host components in a dose-dependent manner were Lp21, Lsa30 and Lp22. These proteins adhered to plasma fibronectin and laminin, in addition to these components, Lp21 and Lp22 interacted with plasminogen, Lp22 and Lsa30 interacted with collagen IV. The Lp22 adhered to elastin and fibrinogen. The genes encoding the probable lipoproteins were found only in pathogenic Leptospira. These results demonstrated that these proteins may contribute in the adhesion to host tissues, in the pathogenesis of Leptospira.
 
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Release Date
2016-12-09
Publishing Date
2014-12-10
 
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