• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.87.2008.tde-05012009-103835
Document
Author
Full name
André Luís Braghini Sá
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Melo, Itamar Soares de (President)
Araujo, Welington Luiz de
Pizzirani-kleiner, Aline Aparecida
Title in Portuguese
Diversidade de rizobactérias endoglicolíticas isoladas de mangue vermelho (Rhizophora mangle).
Keywords in Portuguese
Bacillus subtilis
EglA
Biofilme
Endoglicanase
Salinidade
Abstract in Portuguese
Os manguezais são ambientes ricos em biodiversidade, cuja funcionalidade reside na ciclagem dos nutrientes e seu principal representante vegetal é Rhizophora mangle. Este estudo objetivou conhecer a diversidade bacteriana endoglicolítica e a tolerância à salinidade de rizobactérias associadas à R. mangle. Das amostras de plantas do manguezal de Bertioga (contaminado com petróleo) e Cananéia (não impactado) isolou-se 129 bactérias, das quais 30 apresentaram atividade endoglicolítica, com Bacillus subtilis isolado 39a como melhor produtor. A presença do gene EglA foi confirmada por amplificação com primers específicos. As linhagens testadas para salinidade mostraram-se halotolerantes, com destaque para o 39a, que cresceu em NaCl 20%. A microscopia eletrônica pós-cultivo em diferentes salinidades mostrou produção de biofilme em concentrações altas. Os resultados indicam que a preservação do ecossistema cria um ambiente bacteriano mais diverso e mostra Bacillus spp. como principal produtor de endoglicanase, além de responder ao stress salino formando biofilme.
Title in English
Diversity of endoglucolytic rhizobacteria isolated from red mangrove (Rhizophora mangle).
Keywords in English
Bacillus subtilis
EglA
Biofilm
Endoglucanase
Salinity
Abstract in English
Mangroves are environments so rich in biodiversity which functionality made by nutrient cycling. The main vegetable specie is Rhizophora mangle. This study objected to know bacterial endoglucolytic diversity and tolerance saline of rhizobacteria associated to R. mangle. Plants from Bertioga (oil contaminated) and Cananéia (not polluted) were sampled. From both sites, 129 bacteria were isolated, which most diversity observed from Cananéia. These isolates, 30 presented endoglucolytic activity and Bacillus subtilis (strain 39a) was characterized as top producer. The presence of EglA gene was confirmed using specific primers. The salinity test showed halotolerance, mainly strain 39a that growth untill about 20% NaCl. The scan electron microscopy of strains allowed biofilm production at elevated salinity that suggest the biofilm as tolerance mechanism to saline environment. The results indicated that ecosystem preservation makes a most diversity bacterial environment and that Bacillus spp. are main endoglucanase producer and response to saline stress producing biofilm.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2009-02-16
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.