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Master's Dissertation
DOI
10.11606/D.87.2010.tde-01032011-181049
Document
Author
Full name
Ana Claudia Pacheco
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Maria, Durvanei Augusto (President)
Iwamura, Edna Sadayo Miazato
Ruiz, Itamar Romano Garcia
Title in Portuguese
Emprego de miniSTRs "non-CODIS" em amostras biológicas de DNA forense.
Keywords in Portuguese
DNA
Eletroforense capilar de zona
Genética (técnicas)
Identificação (humano)
Investigação criminal
Reação em cadeia por polimerase
Abstract in Portuguese
Foram analisadas 80 amostras de casos forenses criminais do Laboratório de DNA do Instituto de Criminalística de São Paulo. O DNA foi extraído de materiais cadavéricos, vestígios de crimes sexuais e de locais de crimes. A quantificação se deu por PCR em tempo real e a amplificação por PCR, em 2 reações triplex, dos miniSTRs non-CODIS D10S1248, D14S1434 e D22S1045 (NCO01) e D1S1677, D2S441 e D4S2364 (NCO02), com posterior eletroforese capilar para detecção dos produtos fluorescentes. Avaliou-se o grau de conservação das amostras e a qualidade dos perfis genéticos obtidos. Houve concordância nas dificuldades entre diferentes amostras quando comparado com as abordagens tradicionais. Confirmou-se a sensibilidade e robustez dos sistemas utilizados, bem como a vantagem em se aumentar o número de regiões analisadas, principalmente em casos complexos, mas foram constatadas desvantagens de procedimentos tipo in-house. É necessário o estudo das frequências alélicas destes loci para a população brasileira, que possam ser incorporadas nos cálculos estatísticos dos laudos.
Title in English
The use of "non-CODIS" miniSTRS in forensic DNA biological samples.
Keywords in English
Capillary zone eletroforense
Criminal investigation
DNA
Genetics (technical)
Identification (human)
Polymerase chain reaction
Abstract in English
80 forensic casework samples from the DNA Laboratory of the Criminalistics Institute of São Paulo were analyzed. DNA was extracted from corpse, sexual assault and crime scene evidence. The extractions were quantified using real-time PCR, amplified by two triplex PCR reactions of the non-CODIS miniSTRs D10S1248, D14S1434, D22S1045 (NC01) and D1S1677, D2S441, D4S2364 (NC02), followed by capillary electrophoresis to detect the fluorescent products. The sample preservation and quality of the genetic profiles obtained were evaluated. The results were compared to the previous obtained with the routine techniques employed in the laboratory and there was concordance in the relative difficulties. The sensibility and robustness of the systems employed were confirmed, as well as the advantages in increasing the number of loci studied, mainly in complex cases, although the in-house procedures were considered a disadvantage. The establishment of brazilian population allele frequencies for these loci is necessary for the statistical calculations of the forensic reports.
 
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Publishing Date
2011-08-17
 
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