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Doctoral Thesis
DOI
https://doi.org/10.11606/T.85.2021.tde-10032022-144112
Document
Author
Full name
Marcelo José Vigorito Campara
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2021
Supervisor
Committee
Freitas, Anderson Zanardi de (President)
Cechetti, Neuza Maria
Herrera, Cristhiano da Costa
Rossi, Wagner de
Title in Portuguese
Desenvolvimento de uma ferramenta mobile para teste Elisa em microcircuitos e detecção de cores com inteligência artificial
Keywords in Portuguese
clusters
desenvolvimento mobile
microcircuitos microfluídicos
processamento de imagens
Abstract in Portuguese
A análise de materiais é uma área na qual pesquisadores vem se esforçando cada vez mais na busca de ferramentas mais precisas, visando desvendar informações muitas vezes não evidentes sem ajuda de instrumentos. O objetivo principal foi desenvolver um protótipo analisador microfluídico móvel, capaz de avaliar variações de cores em amostras microfluídicas, visando diagnósticos baseados em exames Enzyme-Linked ImmunonoSorbent Assay (ELISA). O mapeamento de cores em microcircuitos proporcionou quatro aplicações diferentes. Primeiramente, contribuindo para análises em exames ELISA executados dentro de microcircuitos microfluídicos. Posteriormente foi utilizada a mesma metodologia para estudar o comportamento do microfluido dentro do microcircuito, usando um aplicativo de celular, baseando-se no modelo Red-Green-Blue (RGB). Ainda foi desenvolvido um software capaz de mapear as cores usando o modelo conhecido como Hue-Saturation-Value (HSV). Por fim, foi desenvolvido um software baseado em uma técnica de inteligência artificial conhecida como agrupamento ou clusters. A base de todas as análises são fotos capturadas dos microcircuitos usando uma interface especialmente projetada em impressão tridimensional (3D). As análises proporcionaram uma nova forma de analisar amostras ELISA e também um novo meio de avaliar o desempenho dos microcircuitos de diferentes formatos, contribuindo de forma direta no design de sua fabricação.
Title in English
Development of a mobile tool for Elisa tests in microcircuits and color detection with artificial intelligence
Keywords in English
clusters
image processing
microfluidic microcircuits
mobile development
Abstract in English
Material analysis is an area in which there has been increasing effort in the search for more accurate tools, aiming to unveil information that is often not evident without the help of instruments. The main objective was to develop a mobile microfluidic analyzer prototype, capable of evaluating color variations in microfluidic samples, aiming at diagnosis based on Enzyme-Linked ImmunoSorbent Assay (ELISA) exams. Color mapping on microcircuits provided four different applications. Firstly, contributing to analysis in ELISA tests performed within microfluidic microcircuits. Subsequently, the same methodology was used to study the behavior of the microfluid inside the microcircuit, using a cell phone application, based on the Red-Green-Blue (RGB) model. A software capable of mapping the colors using the model known as Hue-Saturation-Value (HSV) was also developed. Finally, a software based on an artificial intelligence technique known as clustering or clusters was developed. The basis of all analyzes are captured photos of the microcircuits using a specially designed three-dimensional (3D) print interface. The analyzes provided a new way to analyze ELISA samples and also a new way to evaluate the performance of microcircuits of different formats, directly contributing to the design of their manufacture.
 
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Publishing Date
2022-03-14
 
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