• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
10.11606/T.85.2012.tde-03042012-090522
Document
Author
Full name
Sabrina Metairon
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Zamboni, Cibele Bugno (President)
Madi Filho, Tufic
Oliveira, Maria Regina Andrade de Azevedo
Sant'Anna, Osvaldo Augusto Brazil Esteves
Sato, Ivone Mulako
Title in Portuguese
Determinação de elementos de relevância clínica em tecidos biológicos decamundongos distróficos Dmdmdx/J por AAN
Keywords in Portuguese
análise por ativação com nêutrons
análises bioquímicas
distrofia muscular de Duchenne
tecidos biológicos
valores de referência
Abstract in Portuguese
Neste trabalho a determinação de elementos químicos em tecidos biológicos (sangue total, ossos e orgãos) de camundongos distróficos, usados como modelo animal da Distrofia Mucular de Duchenne (DMD), foi realizada utilizando técnica analítica nuclear. O objetivo do presente trabalho foi a determinação dos valores de referência para elementos de relevância em bioquímica clínica (Ca, Cl, K, Mg e Na) e nutricional (Br e S) em sangue total, tíbia, quadríceps anterior e coração de camundongos da linhagem distrófica Dmdmdx/J (10 machos e 10 fêmeas) e grupo controle C57BL/6J (10 machos), utilizando a técnica de Análise por Ativação com Nêutrons AAN. Para obter mais detalhes das alterações que esta disfunção pode causar nesses tecidos biológicos, foram calculadas matrizes de correlação entre as espécies Dmdmdx/J e comparadas ao grupo controle C57BL/6J. Para a realização deste estudo 119 amostras de tecidos biológicos foram irradiadas no reator nuclear IEA-R1 no IPEN (São Paulo, Brasil). Os resultados de análise, por AAN, constituem as primeiras estimativas para os valores de referência nesses tecidos biológicos dos camundongos Dmdmdx/J e C57BL/6J. Além disso, as alterações em alguns dos coeficientes de correlações entre os animais saudáveis e com disfunção indicam uma conexão entre esses elementos no sangue, tíbia, quadríceps e coração. Esses dados poderão auxiliar pesquisadores avaliar e comparar as vantagens e desvantagens dos diferentes tratamentos, realizados na distrofia muscular, quando estes modelos animais forem empregados, auxiliando os pesquisadores a avaliar a eficácia de novos procedimentos terapêuticos antes de serem empregados em paciente com DMD.
Title in English
Elements determination of clinical relevance in biological tissues of Dmdmdx/j dystrophic mice strains investigated by NAA
Keywords in English
biochemistry analysis
biological tissues
Duchenne Muscular Dystrophy
neutron activation analysis
reference values
Abstract in English
In this work the determination of chemistry elements in biological tissues (whole blood, bones and organs) of dystrophic mice, used as animal model of Duchenne Muscular Dystrophy (DMD), was performed using analytical nuclear technique. The aim of this work was to determine reference values of elements of clinical (Ca, Cl, K, Mg, Na) and nutritional (Br and S) relevance in whole blood, tibia, quadriceps and hearts from Dmdmdx/J (10 males and 10 females) dystrophic mice and C57BL/6J (10 males) control group mice, using Neutron Activation Analysis technique (NAA). To show in more details the alterations that this disease may cause in these biological tissues, correlations matrixes of the DMDmdx/J mouse strain were generated and compared with C57BL/6J control group. For this study 119 samples of biological tissue were irradiated in the IEA-R1 nuclear reactor at IPEN (São Paulo, Brazil). The concentrations of these elements in biological tissues of Dmdmdx/J and C57B/6J mice are the first indicative interval for reference values. Moreover, the alteration in some correlation coefficients data among the elements in the health status and in the diseased status indicates a connection between these elements in whole blood, tibia, quadriceps and heart. These results may help the researchers to evaluate the efficiency of new treatments and to compare the advantages of different treatment approaches before performing tests in patients with muscular dystrophy.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2012-04-04
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.