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Doctoral Thesis
DOI
10.11606/T.75.2012.tde-24072012-164610
Document
Author
Full name
Matheus Pereira Postigo
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2012
Supervisor
Committee
Colnago, Luiz Alberto (President)
Cardoso, Daniel Rodrigues
Azeredo, Rodrigo Bagueira de Vasconcellos
Pereira Filho, Edenir Rodrigues
Thiemann, Otavio Henrique
Title in Portuguese
Uso de ressonância magnética nuclear na análise metabolômica de biofluidos de animais tratados com ivermectina
Keywords in Portuguese
metaboloma
ressonância magnética nuclear
SSFP
Abstract in Portuguese
A pesquisa bioquímica no campo da Metabolômica/Metabonômica tem se intensificado consideravelmente nos últimos anos, por sua capacidade de adquirir uma grande quantidade de informação a respeito do comportamento de um organismo através de seu metabolismo. Para isso, frequentemente faz uso da aplicação das mais diversas técnicas analíticas, como a Ressonância Magnética Nuclear. A Ivermectina é um fármaco de amplo uso no Brasil, dada a sua eficiência no controle de verminoses e pragas em gado (e humanos) e está aqui inserida no contexto metabolômico/metabonômico dadas as inúmeras violações ocorridas na carne brasileira exportada. A não observância dos períodos adequados de carência para abate dos animais tratados pode refletir seriamente na qualidade destes produtos. Assim, utilizou-se a Ivermectina como forma de provocar mudanças no metabolismo de bovinos e camundongos, procurando-se correlacionar as variações encontradas à dose aplicada. Através de ferramentas auxiliares, como RMN-2D e ferramentas quimiométricas exploratórias, fez-se a avaliação de amostras de plasma sanguíneo e urina bovinos, e plasma sanguíneo de camundongos Balb-C, após administração de Ivermectina. Os resultados obtidos mostram que a Ivermectina tem influência no balanço energético do organismo, interferindo nos níveis de lactato e β-hidróxibutirato, podendo estar ligada ao aparecimento de uma condição metabólica crítica em mamíferos, relacionada à alta concentração de corpos cetônicos na corrente sanguínea dos mesmos.
Title in English
Use of Nuclear Magnetic Resonance in metabolomic analysis of biofluids from animails trated with Ivermectin
Keywords in English
Metabolomics
Nuclear Magnetic Resonance
SSFP
Abstract in English
The biochemical research in the field of Metabolomics/ Metabonomics has grown considerably in recent years because its capability of acquiring a large amount of information about the behavior of an organism through its metabolism. For this, it often applies several analytical techniques such as Nuclear Magnetic Resonance. Ivermectin is a drug widely used in Brazil, for its effectiveness in controlling verminosis and pests in livestock (and humans) and is here inserted in the metabolomic/metabonomic context because of the numerous breaches occurred in brazilian beef exports. Failures to comply with the appropriate withdrawal periods for slaughtering treated animals may reflect seriously on the quality of these products. Thus, we used Ivermectin as a metabolism change inducer in cattle and mice, trying to correlate these variations to the applied dose. Through auxiliary tools such as 2D-NMR and chemometric exploratory tools, we evaluated samples of bovine blood plasma and urine, and blood plasma of Balb-C mice, after Ivermectin administration. The results show that Ivermectin has influence on the organism's energy balance, interfering with lactate and β-hydroxybutyrate which can be connected to the onset of a critical metabolic condition in mammals, related to the high concentration of ketone bodies in their blood stream.
 
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Publishing Date
2012-09-21
 
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