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Master's Dissertation
DOI
10.11606/D.74.2019.tde-14022019-132753
Document
Author
Full name
Thiago Henrique Moroni Vargas
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Pirassununga, 2018
Supervisor
Committee
Strefezzi, Ricardo de Francisco (President)
Ferro, Daniel Giberne
Fukumasu, Heidge
Luvizotto, Maria Cecilia Rui
Title in Portuguese
Caracterização imuno-histoquímica da Galectina-3 como ferramenta prognóstica em melanomas orais caninos
Keywords in Portuguese
Apoptose
Galectina 3
Melanoma
Núcleo
Prognóstico
Abstract in Portuguese
Os melanomas correspondem a 7% de todas as neoplasias malignas em cães e são principalmente encontrados em cavidade oral e lábios, correspondendo a 33% dos tumores de boca, possuem um prognóstico ruim devido ao fato de serem diagnosticados tardiamente, por sua grande capacidade de invasão local e formação de metástases, além de altas taxas de recidiva após o tratamento cirúrgico. A Galectina-3 (Gal-3) é uma proteína responsável por diversas funções fisiológicas como adesão, apoptose, angiogênese, proliferação e diferenciação. Em medicina veterinária existem poucos estudos relacionando à expressão da Gal-3 com prognóstico e a progressão da neoplasia. Realizamos imuno-histoquímica para Gal-3 em 27 melanomas orais caninos que foram avaliados de maneira semiquantitativa e quantitativa, e comparamos os resultados obtidos com a sobrevida, outros marcadores prognósticos (Ki67, índice mitótico e atipia nuclear), expressão de proteínas relacionadas à apoptose (BCL2 e CASP3) e parâmetros histopatológicos (grau de pigmentação e tipo histológico). Detectamos alta expressão de Gal-3 em melanomas com maior sobrevida pós-cirúrgica e uma alta expressão nuclear de Gal-3 em melanomas com menor sobrevida pós-cirúrgica. Além disso, houve correlação entre as expressões de Gal-3 e BCL2, assim como entre atipia nuclear e sobrevida pós-cirúrgica. É sabido que a Gal-3 é capaz de formar heterodímeros com a BCL2 no citoplasma para atuar na evasão da morte por apoptose, impedindo a liberação da citocromo C. Já no núcleo, a Gal-3 induz à parada do ciclo celular, reduzindo a taxa da proliferação. Apesar do reduzido número amostral devido à dificuldade nos acompanhamentos clínicos nossos dados permitem sugerir que a Gal-3 é possui potencial para ser um marcador prognóstico de sobrevida em casos de melanomas orais caninos. Novos estudos devem ser realizados afim de confirmar nossas observações e elucidar o papel da Gal-3 nesta neoplasia.
Title in English
Immunohistochemical characterization of Galectin-3 as prognostic tool in canine oral melanomas
Keywords in English
Apoptosis
Galectin 3
Melanoma
Nuclei
Prognostic
Abstract in English
Melanomas are almost 7% of all malignant neoplasms in dogs. They are mainly found in the oral cavity and lips, corresponding to 33% of tumors of the oral cavity. They carry a poor prognosis because of late diagnoses, local invasiveness, high metastatic and recurrence rates after surgical treatment. Galectin-3 (Gal-3) is a protein with a variety of biological roles such as in adhesion, apoptosis, angiogenesis, proliferation and differentiation. In veterinary medicine, there are few studies comparing the expression of Gal-3 with prognosis and tumor progression. We performed immunohistochemistry for Gal-3 in 27 canine oral melanomas and evaluated the immunolabelling both semi-quantitatively and quantitatively. The results were compared with survival, other prognostic markers (Ki67, mitotic index and nuclear atypia), expression of proteins related to apoptosis (BCL2 and CASP3) and histopathological parameters (degree of pigmentation and histological type). We detected higher expression of Gal-3 in cases of melanoma that presented longer post-surgical survival and a higher nuclear expression of Gal-3 in dogs with melanoma that had shorter post-surgical survival. In addition, there was correlation between the Gal-3 and BCL2 expressions, as well as between nuclear atypia and post-surgical survival. It is known that Gal-3 is able to form heterodimers with BCL2 in the cytoplasm leading to evasion of apoptosis, through preventing mitochondrial cytochrome C release. Nuclear Gal-3 induces the cell cycle arrest, reducing the proliferation rate. Despite the small sample size due to the difficulty in clinical follow-up, our data suggest that Gal-3 has the potential to be a prognostic marker for survival in cases of canine oral melanomas. Further studies should be performed to confirm our observations and elucidate the role of Gal-3 in this neoplasm.
 
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ME7145492COR.pdf (3.76 Mbytes)
Publishing Date
2019-03-08
 
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