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Master's Dissertation
DOI
https://doi.org/10.11606/D.74.2022.tde-04072023-094923
Document
Author
Full name
Thaís Naliato Chequer
Institute/School/College
Knowledge Area
Date of Defense
Published
Pirassununga, 2022
Supervisor
Committee
Vidal, Ana Maria Centola (President)
Souza, Bruna Maria Salotti de
Telles, Evelise Oliveira
Title in Portuguese
Quantificação da Betacasomorfina-7 após processamento tecnológico de leite oriundo de vacas com alelos contrastantes para β-caseína
Keywords in Portuguese
BCM-7
Beta-CN
Derivados lácteos
HPLC
Leite A2
Peptídeos opioides
Abstract in Portuguese
A comunidade científica ainda não está totalmente de acordo quanto aos benefícios do consumo de leite oriundo de vacas com genótipo A2A2, em relação ao A1A1. Considerando que as novas demandas do mercado consumidor estão cada vez mais relacionadas à qualidade dos nutrientes e aos benefícios aportados à saúde e o bem-estar, a identificação e quantificação da BCM-7 em leite e derivados abre caminhos para a popularização do leite A2 e serve de base para outros estudos na área. Esses peptídeos são derivados a partir da β-caseína do leite e demonstram diversas atividades opióides e farmacológicas. As BCM-7 se ligam a receptores, que estão localizados no sistema nervoso central, trato gastrointestinal e algumas células imunes; podendo desencadear algumas doenças. Este estudo teve a finalidade de quantificar a BCM-7 no leite cru e pasteurizado, bem como em seus derivados (iogurte e queijo minas frescal) produzidos com leite oriundo de vacas com genótipos A1A1 e A2A2 para β-CN, ao longo da vida de prateleira de modo a averiguar se os tratamentos industriais têm influência na liberação desse peptídeo bioativo, bem como comparar a disponibilidade de BCM-7 em diferentes genótipos. As amostras foram lidas através do HPLC e foi possível verificar maior abundância desse peptídeo no genótipo A1A1, porém não observou ausência de BCM-7 no genótipo A2A2.
Title in English
Quantification of Beta casomorphin-7 after technological milk processing of cows with contrasting alleles for β-casein
Keywords in English
A2 milk
BCM-7
Beta-casein
Dairy products
HPLC
Opioid peptides
Abstract in English
The scientific community still does not fully agree on the benefits of consuming milk from cows with A2A2 genotype, as compared to A1A1. Considering that the new demands of the consumer market are increasingly related to the quality of the nutrients and the benefits provided to health and well-being, the identification and quantification of BCM-7 in milk and dairy products open the way to popularize A2 milk and serve as a basis for further studies in the area. These peptides are derived from the β-casein of milk and demonstrate diverse opioid and pharmacological activities. BCM-7 bind to receptors, which are located in the central nervous system, gastrointestinal tract and some immune cells; they can trigger some diseases. In order to quantify BCM-7 in raw and pasteurized milk, as well as in its derivatives (yogurt and minas frescal cheese) produced with milk from cows with A1A1 and A2A2 genotypes for β-CN, throughout the shelf life in order to investigate whether industrial treatments have an influence on the release of this bioactive peptide, as well as to compare the availability of BCM-7 in different genotypes. For the quantification of Betacasomorphin-7, the samples were read by HPLC and, it was possible to verify a higher abundance of this peptide in the A1A1 genotype, however, no absence of BCM-7 was observed in the A2A2.
 
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ME7276092COR.pdf (1.73 Mbytes)
Publishing Date
2023-07-04
 
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