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Doctoral Thesis
DOI
https://doi.org/10.11606/T.64.2021.tde-26042023-151851
Document
Author
Full name
Joyce Ribeiro Santos Rasera
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2021
Supervisor
Committee
Carvalho, Hudson Wallace Pereira de (President)
Fraceto, Leonardo Fernandes
Lima, Renata de
Perez, Carlos Alberto
Title in English
Evaluation of distribution, chemical speciation, and toxic effects of CuO and ZnO nanoparticles in Daphnia magna and Danio rerio
Keywords in English
CuO nanoparticles
Danio rerio
Daphnia magna
Toxicity
X-ray absorption
X-ray fluorescence
ZnO nanoparticles
Abstract in English
The use of products with nanoparticles has been growing in recent years. Currently, there is the application of nutrients in nanoparticulate form in agriculture. With this growth, it is important to safely expand their use and assess the environmental risk caused by them, as discharges may occur near water flows. In this work, the toxicity, distribution, concentration and chemical speciation of CuO and ZnO nanoparticles in ecotoxicology model organisms, Daphnia magna and Danio rerio were evaluated. Copper and zinc were chosen due to their agronomic importance. In this study, higher toxicity was determined in CuSO4 and in smaller nanoparticles of CuO, for both organisms. For ZnO, the toxicity depended on the particle size and the presence of surfactant, also higher for smaller nanoparticles. The chemical speciation analysis showed that there was biotransformation of CuO (25 nm) and CuSO4 into Cu3(PO4)2 in D. magna; however there was no transformation in D. rerio. The distribution of copper and zinc was similar for organisms, with the highest concentration occurring in the intestinal and gastrointestinal systems for D. magna and D. rerio, respectively. In this study, there was no ZnO clearance in D. magna observed for 24 h
Title in Portuguese
Avaliação da distribuição, especiação química e efeitos tóxicos de nanopartículas de CuO e ZnO em Daphnia magna e Danio rerio
Keywords in Portuguese
Absorção de raios X
Danio rerio
Daphnia magna
Fluorescência de raios X
Nanopartículas de CuO
Nanopartículas de ZnO
Toxicidade
Abstract in Portuguese
O uso de produtos com nanopartículas vem crescendo nos últimos anos. Atualmente, há a aplicação de nutrientes na forma nanoparticulada na agricultura. Com este crescimento, é importante expandir com segurança o seu uso e avaliar o risco ambiental provocada por elas, visto que pode ocorrer descargas próximo a fluxos d´agua. Neste trabalho, foi avaliado a toxicidade, a distribuição, concentração e forma química de nanopartículas de CuO e ZnO em dois organismos modelos de ecotoxicologia - Daphnia magna e Danio rerio. Cobre e zinco foram escolhidos devido a sua importância agronômica. Neste estudo, foi determinado maior toxicidade para nanopartículas menores de CuO e CuSO4 em ambos os organismos. Para ZnO, a toxicidade dependeu do tamanho da partícula e da presença de surfactante, sendo também maior para nanopartículas menores. A análise de especiação química mostrou que ocorreu biotransformação de CuO (25 nm) e CuSO4 em Cu3(PO4)2 em D. magna; entretanto não houve transformação em D. rerio. A distribuição de cobre e zinco foi semelhante em ambos os organismos, sendo que a maior concentração ocorreu no sistema intestinal e gastrointestinal para D. magna e D. rerio, respectivamente. Neste estudo, não houve depuração de ZnO em D. magna observadas por 24 h
 
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Publishing Date
2023-08-31
 
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