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Master's Dissertation
DOI
10.11606/D.60.2012.tde-21052012-111937
Document
Author
Full name
Eloísa Silva de Paula
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2012
Supervisor
Committee
Oliveira, Danielle Palma de (President)
Santos, Antonio Cardozo dos
Santos, Raquel Alves dos
Title in Portuguese
Avaliação da mutagenicidade dos corantes Sudan III, Vat Green 3, Reactive Orange 16 e Reactive Black 5 por meio do ensaio de micronúcleos em células HepG2
Keywords in Portuguese
Células HepG2
Ensaio de micronúcleos
Reactive Black 5
Reactive Orange 16
Sundan III
Vat Green 3
Abstract in Portuguese
As cores sempre exerceram fascínio sobre a humanidade e, por toda a história, os compostos coloridos sempre foram considerados ferramentas atrativas nas atividades comerciais. Os corantes sintéticos são amplamente utilizados na indústria têxtil, nas impressões de papel e fotografia, nas indústrias farmacêuticas, alimentícias e de cosméticos. Estes compostos são considerados importantes contaminantes ambientais, representando sérios riscos à flora, fauna e ao ser humano. Apesar da grande quantidade de corantes disponíveis, os estudos sobre a toxicidade desses compostos são escassos e pouco se sabe a respeito dos efeitos genotóxicos destas substâncias. Dentro deste contexto, o presente trabalho avaliou o potencial genotóxico dos corantes Sudan III, Vat Green 3, Reactive Orange 16 e Reactive Black 5, utilizando o Ensaio de Micronúcleos em células HepG2. Os corantes Sudan III e Reactive Orange 16 não induziram aumento, estatisticamente significativo, no número total de micronúcleos em relação aos controles, indicando assim que estes corantes não são capazes de induzir mutações cromossômicas no tipo celular e condições testadas. Entretanto, os corantes Vat Green 3 e Reactive Black 5 induziram mutagenicidade, concentrações de 10,0 e 25,0 ?g/mL, e 0,1; 0,25; 0,5 e 1,0 ?g/mL, respectivamente, demonstrada por um efeito concentraçãodependente, no qual há um aumento de MNs até a concentração de 25,0 ?g/mL para o Vat Green 3 e 0,5 ?g/mL para o Reactive Black 5 com p<0,05. Não foram observadas diferenças significativas entre os IDNs calculados para cada tratamento e controle dos corantes testados, indicando que esses corantes não interferem na proliferação celular das HepG2. Dessa forma, conclui-se que dos quatro compostos analisados, os corantes têxteis Vat Green 3 e Reactive Black 5 são capazes de induzir mutações cromossômicas em células HepG2 e, o potencial mutagênico do Reactive Black 5 é maior que o do Vat Green 3 no sistema celular avaliado, uma vez que foi capaz de induzir mutações, em concentrações menores. Os resultados obtidos neste trabalho permitem concluir que cada um desses importantes contaminantes ambientais deve ser avaliado individualmente a fim de proteger o meio ambiente, garantindo assim a proteção da saúde humana.
Title in English
Evaluation of the mutagenicity of the dyes Sudan III, Vat Green 3, Reactive Orange 16, and Reactive Black 5 by using the micronucleus asay in HepG2 cells
Keywords in English
HepG2 cells
Micronucleus assay
Reactive Black 5
Reactive Orange 16
Sudan III
Vat Green 3
Abstract in English
The colors have always caused fascination in mankind. Throughout history, colored compounds have always been considered attractive tools in industrial activities. The synthetic dyes are widely used in textile industry, paper and photography printing, in pharmaceutical, food, and cosmetic industries. These compounds are considered important environmental contaminants, and they can cause serious risks to wildlife and humans. Despite the large number of dyes available, studies on the toxicity of these compounds are scarce and little is known about the genotoxic effects of these substances. This study evaluated the genotoxic potential of the dyes Sudan III, Vat Green 3, Reactive Orange 16 and Reactive Black 5 using the micronucleus assay in HepG2 cells. The dyes Sudan III and, Reactive Orange 16, do not induce an increase statistically significant, in the total number of micronuclei when compared to controls. This result shows that these dyes are not able to induce chromosomal mutations in the cell type under the conditions tested. However, the dyes Vat Green 3 and Reactive Black 5 induced mutagenicity, following a dose-response effect, in which there is an increase of micronuclei until the concentration of 25.0 ?g/mL for Vat Green 3 and 0.5 ?g/mL for Reactive Black 5, with p <0.05. There were no significant differences between the NDI calculated for each treatment and control of the dyes studied, indicating that these dyes do not interfere in HepG2 cell proliferation. Thus, the textile dyes Vat Green 3 and Reactive Black 5 are able to induce chromosomal mutations in HepG2 cells, and the dye Reactive Black 5 is more mutagenic than the dye Vat Green 3, since it induced mutations in cellular system tested at lower concentrations. The results of this study indicate that each one of these important environmental contaminants should be assessed individually in order to protect the environment, thus ensuring the protection of human health.
 
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Publishing Date
2012-07-16
 
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