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Master's Dissertation
DOI
10.11606/D.58.2017.tde-15032016-140709
Document
Author
Full name
Sofia Sampaio de Sousa Farias
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2015
Supervisor
Committee
Borsato, Maria Cristina (President)
Aires, Carolina Patricia
Rocha, Cristiane Tomaz
Title in Portuguese
Efeito da terapia fotodinâmica antimicrobiana no controle do biofilme dental cariogênico in vitro
Keywords in Portuguese
Streptococcus mutans
Biofilmes
Laser de diodo
Polissacarídeos
Terapia fotodinâmica
Abstract in Portuguese
O objetivo deste estudo foi avaliar o efeito da terapia fotodinâmica antimicrobiana (TFDa), utilizando laser de baixa potência, em duas densidades de energia, associado ao azul de toluidina em biofilme de S. mutans. Biofilmes de S. mutans UA159 foram crescidos por 5 dias em discos de resina acrílica (3 mm de diâmetro x 2 mm de altura) a 37ºC 5 % de CO2;. Os espécimes foram divididos aleatoriamente em 4 grupos de tratamento (n=3): Digluconato de clorexidina 0,12% (CHX, controle positivo); Solução salina a 0,89% (NaCl, controle negativo); Terapia fotodinâmica antimicrobiana com azul de toluidina e laser de baixa potência (densidade de energia 320 J/cm²) (TFDa 320); Terapia fotodinâmica antimicrobiana com azul de toluidina e laser de baixa potência (densidade de energia 640 J/cm²) (TFDa 640). Os tratamentos foram realizados 2x/dia durante 3 dias. Ao final dos 5 dias, os biofilmes foram coletados e número de bactérias viáveis e a concentração de polissacarídeos extracelulares insolúveis (PECI) e intracelulares (PIC) foram determinadas e analisadas estatisticamente (ANOVA e teste de Tukey, p < 0,05). O tratamento com a TFDa (320 e 640 J/cm²) reduziu o número de bactérias viáveis em biofilmes de S. mutans, de uma maneira dose-dependente (p < 0,05). Além disso, a TFDa 640 J/cm², reduziu a viabilidade bacteriana de forma tão eficaz quanto a CHX (p > 0,05). Em relação a PECI e PIC, os grupos TFDa (320 e 640 J/cm²) não foram significativamente diferentes de CHX (p > 0,05). Os resultados mostraram que a terapia fotodinâmica antimicrobiana, mediada pelo azul de toluidina e laser de diodo (640 J/cm²), pode ser uma abordagem utilizada no controle do biofilme dental cariogênico.
Title in English
Effect of antimicrobial photodynamic therapy in the control of cariogenic dental biofilm in vitro
Keywords in English
Streptococcus mutans
Biofilms
Diode laser
Photodynamic therapy
Polysaccharides
Abstract in English
The aim of this study was to evaluate the effect of antimicrobial photodynamic therapy (aPDT), using low-power laser, with two energy densities, associated with toluidine blue in S. mutans biofilm. S. mutans UA159 biofilms were grown for 5 days on acrylic resin discs (3 mm diameter x 2 mm height) a 37ºC, 5 % de CO2. The specimens were randomly divided into 4 treatment groups (n = 3): 0.12% chlorhexidine digluconate (CHX, positive control); 0,89% Saline (NaCl, negative control); Antimicrobial photodynamic therapy with toluidine blue and low power laser (energy density of 320 J/cm²) (aPDT 320); Antimicrobial photodynamic therapy with toluidine blue and low power laser (energy density of 640 J/cm²) (aPDT 640). Treatments were performed 2x/day for 3 days. At the end of 5 days, the biofilms were collected and the number of viable bacteria and the concentration of insoluble extracellular (IEPS) and intracellular polysaccharides (IPS) were determined and analyzed statistically (ANOVA and Tukey's test, p<0.05). Treatment with aPDT (320 and 640 J/cm²) reduced the number of viable bacteria of S. mutans biofilms in a dose-dependent manner (p<0.05). Furthermore, the aPDT 640 group reduced the bacterial viability as effectively as CHX group (p>0.05). For IEPS and IPS, the aPDT groups (320 and 640 J/cm²) were not significantly different CHX (p>0.05). The results showed that the antimicrobial photodynamic therapy mediated by toluidine blue and diode laser (640 J/cm²) may be an approach used to control the cariogenic dental biofilm.
 
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Publishing Date
2017-08-04
 
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