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Master's Dissertation
DOI
https://doi.org/10.11606/D.58.2020.tde-03102022-180129
Document
Author
Full name
Laís Lima Pelozo
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2020
Supervisor
Committee
Gabriel, Aline Evangelista de Souza (President)
Cruz Filho, Antonio Miranda da
Alfredo, Edson
Salvador, Sergio Luiz de Souza
Title in Portuguese
Estudo clínico longitudinal do uso do laser diodo 980 nm no retratamento dos canais radiculares: efeito antimicrobiano e no reparo ósseo periapical
Keywords in Portuguese
Desinfecção
Endodontia
Estudo clínico
Laser
Periodontite apical
Abstract in Portuguese
Este estudo avaliou o efeito do laser diodo 980 nm na desinfecção dos canais e no reparo ósseo de dentes com indicação de retratamento endodôntico. Trinta pacientes com um dente unirradicular com lesão apical foram selecionados de acordo com os critérios de inclusão. Os canais foram desobturados e foi realizada a 1ª coleta microbiológica com cones de papel e limas. Quinze dentes (n=15) foram alocados para o grupo experimental que recebeu o laser diodo (1,5W, 10Hz, 20s), seguido da 2ª coleta. O preparo biomecânico convencional foi realizado com sistema rotatório (Race/FKG) e hipoclorito de sódio 1%, seguido da nova aplicação de laser e 3ª coleta. O grupo controle (n=15) recebeu o mesmo tratamento do grupo experimental, no entanto, a fibra óptica não foi ligada (placebo). Realizou-se medicação intracanal e restauração provisória com CIV. Após 15 dias, a obturação foi realizada com cones de guta percha e cimento AH Plus. As amostras coletadas, nos diferentes períodos, foram levadas para processamento microbiológico em meio MM10 (contagem geral) e Agar-Enterococus (E. faecalis). O reparo ósseo periapical foi analisado radiograficamente por 3 examinadores (Kappa > 0,8) nos períodos: inicial, 3 e 6 meses. Os dados microbiológicos (log10-UFC/mL) foram analisados por análise de variância de medidas repetidas e teste de Tukey, e o reparo ósseo, pelo teste de Mann-Whitney (p<0,05). Na contagem geral, a menor quantidade de microorganismos foi encontrada após o preparo convencional + laser placebo (1,112 ± 0,833 a), preparo convencional + laser experimental (1,154 ± 1,041 a), e irradiação com laser experimental isoladamente (1,352 ± 0,844 a). A maior quantidade de micro-organismos foi detectada após baseline placebo (2,356 ± 1,034 b), baseline experimental (2,349 ± 0,736 b) e laser placebo (2,395 ± 1,098 b). Para a contagem de E. faecalis, a menor quantidade de micro-organismos foi encontrada após preparo convencional + laser placebo (0,528 ± 0,898 a) e preparo convencional + laser experimental (0,487 ± 0,976 a). Valores intermediários foram encontrados após irradiação com laser experimental isolado (0,917 ± 1,145 ab). A maior quantidade de E. faecalis foi detectada após baseline placebo (1,898 ± 1,186 b), baseline experimental (1,957 ± 1,219 b) e laser placebo (1,806 ± 1,370 b). Com relação ao reparo ósseo, verificou-se que aos 3 meses, não houve diferença significante entre os grupos placebo e experimental e, aos 6 meses, o grupo irradiado obteve melhores resultados de reparo. Pode-se concluir que a irradiação do laser diodo 980 nm durante o retratamento teve efeito antimicrobiano nos canais, porém não foi superior ao preparo biomecânico convencional. O laser diodo favoreceu o reparo de tecido ósseo aos 6 meses após o retratamento.
Title in English
Longitudinal clinical trial of 980 nm diode laser irradiation in canals retreatment cases: antimicrobial effect and periapical bone repair
Keywords in English
Apical periodontitis
Clinical study
Disinfection
Endodontics
Laser
Abstract in English
This study evaluated the 980 nm diode laser effect in root canal disinfection and in periapical bone repair of teeth requiring endodontic retreatment. Thirty patients with one single-rooted tooth with apical periodontitis were selected according to the inclusion criteria. The root canal filling material was removed and the first microbiological sample was taken with paper points and K-files. Fifteen teeth (n=15) were allocated to the experimental group that received diode laser irradiation (1,5W, 10Hz, 20s) followed by the second sample. Conventional biomechanical preparation was performed with rotary NiTi RaCe files and 1% sodium hypochlorite followed by new laser irradiation and the third sample. The control group (n=15) received the same treatment of experimental groups, however, the laser irradiation was simulated without light emission (placebo). Intracanal medication and temporary restoration were done with calcium hydroxide and glass-ionomer cement. After 15 days, the root canal system was filled with gutta-percha and AH Plus sealer. The samples collected, in the different periods, were submitted to the microbiological processing in MM10 (overall counting) and Enterococcus Selective Agar Plates (E. faecalis). The periapical bone repair was radiographically analyzed by 3 examiners (Kappa > 0.8) for the periods: initial, 3 and 6 months. Microbiological data (log10-UFC/mL) were analyzed by analysis of variance of repeated measures and Tukey test and bone repair, using the Mann-Whitney test (p<0.05). In the overall counting, the smallest amount of microorganisms was found after conventional preparation + placebo laser (1.112 ± 0.833 a), conventional preparation + experimental laser (1.154 ± 1.041 a) and isolated experimental laser irradiation (1.352 ± 0.844 a). The largest amount of microorganisms was found after baseline placebo (2.356 ± 1.034 b), baseline experimental (2.349 ± 0.736 b) and placebo laser (2.395 ± 1.098 b). In the E. faecalis counting, the smallest quantity of microorganisms was found after conventional preparation + placebo laser (0.528 ± 0.898 a) and conventional preparation + experimental laser (0.487 ± 0.976 a). Intermediate values were observed after isolated experimental laser irradiation (0.917 ± 1.145 ab). The largest amount of E. faecalis was found after baseline placebo (1.898 ± 1.186 b), baseline experimental (1.957 ± 1.219 b) and placebo laser (1.806 ± 1.370 b). In the analysis of periapical repair at 3 months, it was found no difference in the apical healing between placebo and experimental groups. At 6 months, the laser-irradiated group exhibited better repair results. It may be conclude that 980 nm diode laser irradiation during retreatment had antimicrobial effect on root canals, however, not superior than conventional biomechanical preparation. Diode laser favored bone repair at 6 months after retreatment.
 
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Publishing Date
2022-10-04
 
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