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Master's Dissertation
DOI
10.11606/D.5.2005.tde-19102005-131652
Document
Author
Full name
Ciliane Matilde Sollitto
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Pereira, Luiz Alberto Amador (President)
Martins, Maria Cristina Haddad
Nascimento, Luiz Fernando Costa
Title in Portuguese
"Efeitos clastogênicos em Tradescantia (Trad-MCN) induzidos por campos magnéticos de freqüência extremamente baixa (ELF)"
Keywords in Portuguese
BIOENSAIOS
CAMPOS ELETROMAGNÉTICOS
MONITORAMENTO AMBIENTAL
POLUIÇÃO AMBIENTAL
TESTES DE MUTAGENICIDADE
TESTES PARA MICRONÚCLEOS
TRADESCANTIA
Abstract in Portuguese
Os efeitos clastogênicos de campos magnéticos de freqüência extremamente baixa (ELF) foram investigados usando o bioensaio Tradescantia pallida (Trad-MCN). Inflorescências expostas durante 8 horas a doses de ELF-416mG, 833mG, e 4160mG, prescritas para exposição do público e ocupacional, apresentaram freqüência de micronúcleos aumentada no grupo que recebeu a maior dose (10.32±7.31), comparado com os demais (p=0.00, Kruskal-Wallis). Foi demonstrado que a exposição à ELF, induz danos ao DNA de Tradecantia. Estes resultados encorajam o uso deste bioensaio para avaliar a utilidade do monitor em estudos de campo, auxiliando na caracterização da função do ELF como causa de neoplasmas
Title in English
Extremely low frequency magnetic fileds (ELF) induce clastogenic effects in Tradescantia (Trad-MCN)
Keywords in English
BIOLOGICAL ASSAY
ELECTROMAGNETIC FIELDS
ENVIRONMENTAL MONITORING
ENVIRONMENTAL POLLUTION
MICRONUCLEUS TESTS
MUTAGENICITY TESTS
TRADESCANTIA
Abstract in English
The clastogenic effects of the extremely low frequency magnetic fields (ELF) were investigated using Tradescantia pallida micronucleus assay (Trad-MCN). Inflorescences exposed during 8 hours to doses of ELF-416mG, 833mG, e 4160mG, prescribed for public and occupational exposures, showed increased frequency of micronuclei in the group receiving the highest dose (10.32±7.31), compared with others (p=0.00, Kruskal-Wallis). We demonstrated that an ELF exposure induces DNA damage in Tradescantia. This results encourages the use of this assay to evaluate the usefulness of this monitor in field studies, helping to characterize the role of ELF in causing neoplasm
 
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Publishing Date
2005-10-20
 
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