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Master's Dissertation
DOI
10.11606/D.5.2006.tde-07082006-110753
Document
Author
Full name
Fabiana Baroni Alves Makdissi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Folgueira, Maria Aparecida Azevedo Koike (President)
Barros, Alfredo Carlos Simoes Dornellas de
Maciel, Maria do Socorro
Title in Portuguese
"Análise da expressão de E-caderina, Snail e Hakai em células epiteliais de tumor e tecido peritumoral de mulheres com carcinoma ductal invasivo da mama: correlação com comprometimento linfonodal"
Keywords in Portuguese
Carcinoma ductal de mama
Células epiteliais
Expressão gênica
Linfonodos
Mulheres
Neoplasias mamárias
Abstract in Portuguese
A expressão de E-caderina (Ecad) pode ser regulada pré ou pós transcricionalmente por Snail e Hakai, respectivamente. Nosso objetivo foi determinar a expressão de Ecad, Snail e Hakai, em células epiteliais (CE) de tecido tumoral e peritumoral de pacientes com carcinoma ductal invasivo da mama e correlacionar sua expressão ao comprometimento linfonodal axilar (LN+). As CE de amostras de tecidos de 45 pacientes (52% LN+) foram extraídas por método imunomagnético, o RNA foi extraído por RT-PCR em tempo real e utilizou-se primers específicos para a moléculas. A expressão de Ecad, Snail e Hakai não variou entre o tecido tumoral e peritumoral e não houve correlação com comprometimento linfonodal
Title in English
E-cadherin, Snail and Hakai mRNA expression in epithelial cells from tumoral and peritumoral tissue from women with breast invasive ductal carcinoma. Correlation with axillary's lymph node involvement
Keywords in English
Breast neoplasms
Carcinoma Ductal Breast
Epithelial cells
Gene expression
Lymph nodes
Women
Abstract in English
E-cadherin (Ecad) expression may be transcriptionally or post-transcriptionally regulated by Snail and Hakai. Our aim was to determine the expression of Ecad, Snail and Hakai, in epithelial cells (EC) obtained from tumor and its adjacent tissue from women with invasive ductal breast carcinoma (IDC) and evaluate their correlation to the axillary's lymph node (LN+) involvement. Tissue from 45 patients (52% LN+) had their EC recovered by immunomagnetic antibody process, RNA was extracted and real-time RT-PCR was performed using specific primers. Ecad, Snail and Hakai mRNA expression did not vary between tumoral and peritumoral samples and their expression was not correlated to LN involvement
 
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Publishing Date
2006-08-08
 
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