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Doctoral Thesis
DOI
10.11606/T.5.2008.tde-24032009-164452
Document
Author
Full name
Kátia Galeão Brandt
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Sampaio, Magda Maria Sales Carneiro (President)
Guinsburg, Ruth
Jacob, Cristina Miuki Abe
Martinez, Marina Baquerizo
Silva, Giselia Alves Pontrs da
Title in Portuguese
Análise molecular da microbiota fecal de recém-nascidos saudáveis
Keywords in Portuguese
Instestinos/microbiologia
Reação em cadeia da polimerase
Recém-nascido
Técnica de tipagem bacteriana
Abstract in Portuguese
Objetivo: Analisar através de metodologia molecular a microbiota fecal de recém-nascidos (RN) saudáveis, em aleitamento materno exclusivo. Materiais e métodos: Amostras fecais de dez RN foram avaliadas no 2º, 7º e 30º dias de vida (DV), através de sequenciamento do 16S rDNA bacteriano. Real-time PCR para bifidobacterias foi empregado nas amostras de 30 dias. Resultados: A diversidade bacteriana fecal aumentou do 2º para o 30º DV. E. coli predominou no 2º e 7º DV, e Clostridium no 30º DV. Usando real-time PCR, bifidobacterias foram identificadas em todas as amostras de 30 dias. Conclusão: Enterobacterias predominaram na primeira semana de vida. Aos 30 DV observou-se uma maior diversidade bacteriana, com predomínio de Clostridium.. A técnica inicial não permitiu identificar bifidobacterias.
Title in English
Molecular analysis of fecal microbiota from healthy newborns
Keywords in English
Bacterial typing technique
Intestine/microbiology
Neonate
Polymerase chain reaction
Abstract in English
Purpose: To evaluate by molecular methodology the fecal microbiota of healthy newborns, exclusively breastfed. Materials and methods: Fecal samples from ten neonates were analyzed on 2nd, 7th and 30th day of life, using 16S rDNA sequencing and real-time PCR for bifidobacteria. Results: The fecal bacteria diversity increased from the second to the 30th day of life. E. coli was predominant in the fecal samples from the 2nd and 7th day of life, and Clostridium.in the samples of the 30th day. Using real-time PCR bifidobacteria were identified in all 30th day samples. Conclusion: Enterobacteria were predominant in the first week of life. On 30th day of life a greater bacterial diversity was observed with predominance of Clostridium. The initial technique didnt allow the identification of bifidobacteria.
 
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katiaGBrandt.pdf (1.40 Mbytes)
Publishing Date
2009-03-26
 
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