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Doctoral Thesis
DOI
10.11606/T.5.2006.tde-16082006-155827
Document
Author
Full name
Lucia Maria Almeida Braz
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Okay, Thelma Suely (President)
Doria Filho, Ulysses
Fragata Filho, Abílio Augusto
Netto, Vicente Amato
Ramos, Sonia Regina Testa da Silva
Title in Portuguese
"Comparação da reação em cadeia da polimerase com o exame microscópico na detecção do Trypanosoma cruzi em amostras procedentes do trato digestório do Triatoma infestans"
Keywords in Portuguese
Conteúdo gastrointestinal
Doença de chagas/diagnóstico
Microscopia
Reação em cadeia da polimerase
Triatoma
Trypanosoma cruzi
Abstract in Portuguese
O presente estudo comparou a PCR com o exame microscópico (EM) na detecção do T. cruzi em amostras do trato digestório do T. infestans, do 1º ao 5º, 10º, 15º, 20º, 25º, 30º e 60º dia, após repasto sanguíneo em camundongos Balb/C infectados pelo T. cruzi, divididos em grupos de alta e baixa parasitemia. Concluiu-se que a PCR apresentou vantagens em termos diagnósticos para a doença de Chagas quando comparada ao EM, em 10 dos 11 tempos estudados no grupo de alta parasitemia, e em todos os tempos na baixa parasitemia. O diagnóstico precoce na baixa parasitemia é de relevância clínica já que poderá influenciar o prognóstico dos pacientes imunossuprimidos com reativação da doença de Chagas
Title in English
Comparison of Polymerase Chain Reaction with Microscopic Examination to detect Trypanosoma cruzi in Triatoma infestans digestive tract samples
Keywords in English
Chagas disease/diagnosis
Gastrointestinal contents
Microscopy
Polymerase chain reaction
Triatoma
Trypanosoma cruzi
Abstract in English
Detection of T. cruzi in T. infestans digestive tract samples was compared by PCR and microscopic examination (ME) on the 1st to 5th, 10th, 15th, 20th, 25th, 30th and 60th day after feeding in Balb/C mice infected by T. cruzi, and divided in two groups: with high parasitemia and with low parasitemia. PCR presented vantages to the Chagas' disease diagnosis when compared with EM in 10 of 11 times in high parasitemia, and in all times in low parasitemia. Earlier diagnosis in low parasitemia model is of clinical relevance since may influence prognosis of immunosupressed patients in reactivation of Chagas' disease
 
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Publishing Date
2006-08-21
 
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