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Doctoral Thesis
DOI
10.11606/T.5.2009.tde-25062009-091512
Document
Author
Full name
José Sandro Pereira da Silva
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Croci, Alberto Tesconi (President)
Camargo, Olavo Pires de
Etchebehere, Mauricio
Pécora, José Ricardo
Pedrinelli, André
Title in Portuguese
Estudo das características físico-químicas e biológicas pela adesão de osteoblastos em superfícies de titânio modificadas pela nitretação em plasma
Keywords in Portuguese
Aderência celular
Osteoblastos
Propriedades de superfície
Titânio
Abstract in Portuguese
INTRODUÇÃO: Superfícies de titânio modificadas por diferentes métodos foram estudadas com base nos parâmetros físicos e químicos de caracterização superficial e sua influência no comportamento de células pré-osteoblásticas (MC3T3) in vitro. MÉTODOS: Discos de titânio comercialmente puro grau II foram submetidos a três métodos de modificação de superfície (polimento, nitretados em plasma em configuração planar e gaiola catódica). As diferentes superfícies foram caracterizadas para observar o efeito do processamento na estrutura da camada superficial, na rugosidade e molhabilidade. Ensaios de adesão e proliferação celular usando linhagens de células pré-osteoblásticas MC3T3 foram realizados para avaliar o efeito das novas superfícies no comportamento celular in vitro. RESULTADOS: Os resultados demonstraram que a nitretação em plasma na configuração de gaiola catódica produz superfícies mais rugosas (p<0,02) e com menores ângulos de contato com a água. CONCLUSÕES: A adesão celular é maior nas superfícies mais rugosas do que nas superfícies polidas (p<0,05) e reagem de modo diferente a composição química do substrato e à topografia da superfície.
Title in English
Study of physical-chemical and osteoblast adhesion characteristics of titanium surfaces modified by plasma nitriding
Keywords in English
Cellular adhesion
Osteoblasts
Surface characterization
Titanium
Abstract in English
PURPOSE: The aim of this study was to evaluated the physico-chemical properties of different titanium surfaces modified by means of low temperature plasma nitridind on rat osteoblast cell adhesion and proliferation. METHODS: Pure Titanium discs grade II was submitted to three different surface preparations (polishing, glow discharge plasma nitriding in planar and cathodic cage configurations). Surface parameters as roughness, wettability and chemichal composition was determined to compare influency of gas mixture on the modified surface material properties. Cellular morphology was observed by scanning electron microscopy. To evaluate the effect of the surface on cellular response, osteoblast cells (MC3T3) adhesion and proliferation was quantified and data analised by Kruskal-Wallis and Friedman statistical tests. RESULTS: plasma nitriding discs shows rougher surfaces( p<0,02) in cathodic cage configuration and lower contact angle values. MC3T3 cells attached on rough surfaces produced by cathodic cage configuration was statistically significant p<0,05 compared to polished discs. CONCLUSIONS: Glow discharge plasma nitriding improve titanium surface roughness and wettability. MC3T3 cell adhesion behavior is related to substrate chemical composition and topography.
 
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Tese_JSandro.pdf (2.66 Mbytes)
Publishing Date
2009-07-03
 
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