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Doctoral Thesis
DOI
10.11606/T.5.2005.tde-08102014-094043
Document
Author
Full name
Fernando Luiz Affonso Fonseca
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Giglio, Auro Del (President)
D'Almeida, Vania
Gebrin, Luiz Henrique
Girão, Manoel João Batista Castello
Odone Filho, Vicente
Title in Portuguese
Pesquisa de instabilidade gênica induzida por quimioterapia antineoplásica nas células mononucleares do sangue periférico de mulheres com câncer de mama
Keywords in Portuguese
Leucócitos mononucleares/análise
Neoplasias mamárias/imunologia
Neoplasias mamárias/patologia
Quimioterapia adjuvante/efeitos adversos
Abstract in Portuguese
O tratamento sistêmico é extremamente importante na abordagem clínica do câncer de mama. O presente estudo teve a finalidade de avaliar se a quimioterapia sistêmica é capaz de produzir instabilidade genética representada por instabilidade de microssatélites (MSI) e perda de heterozigosidade (LOH) na fração mononuclear do sangue periférico de pacientes portadoras de câncer de mama. Foram estudadas 119 amostras de sangue, obtidas seqüencialmente antes, durante e após a quimioterapia. Das 30 pacientes avaliadas, 27 apresentaram MSI em pelo menos uma das amostras estudadas e 6 desenvolveram LOH. Uma importante correlação foi obtida entre o número de eventos de MSI por amostra e quimioterápicos com agentes alquilantes (p < 0,0001). Entretanto, quando as amostras foram de pacientes em radioterapia, observou-se um menor número de eventos de MSI por amostra (p=0,038). Concluímos que o tratamento sistêmico pode induzir MSI e LOH em células mononucleares do sangue periférico de pacientes em tratamento quimioterápico com agentes alquilantes
Title in English
Systemic chemotherapy induces microsatellite instability in the peripheral blood mononuclear cells of breast cancer patients
Keywords in English
Breast neoplasms/immunology
Breast neoplasms/pathology
Chemotherapy adjuvant/adverse effects
Leukocytes mononuclear/analysis
Abstract in English
Systemic chemotherapy is an important part of treatment for breast cancer. We conduced the present study to evaluate whether systemic chemotherapy could produce microsatellite instability (MSI) in the peripheral blood mononuclear cell fraction of breast cancer patients. We studied 119 sequential blood samples from 30 previously untreated breast cancer patients before, during and after chemotherapy. In 27 out of 30 patients we observed MSI in at least one sample, and six patients had loss of heterozygosity (LOH). We found a significant correlation between the number of MSI events per sample and chemotherapy with alkylating agents (p < 0.0001). However, when the samples were obtained in patients with radiotherapy we observed low MSI events per samples (p = 0.038). We concluded that systemic chemotherapy may induce MSI and LOH in peripheral blood mononuclear cells from breast cancer patients receiving alkylating agents
 
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Publishing Date
2014-10-08
 
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