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Doctoral Thesis
Full name
Priscila de Arruda Trindade
Knowledge Area
Date of Defense
São Paulo, 2005
Levin, Anna Sara Shafferman (President)
Barone, Antonio Alci
Branchini, Maria Luiza Moretti
Medeiros, Eduardo Alexandrino Servolo de
Pignatari, Antonio Carlos Campos
Title in Portuguese
"Caracterização molecular da resistência a oxacilina em isolados de Staphylococcus aureus hospitalares e comunitários"
Keywords in Portuguese
Epidemiologia molecular
Infecção hospitalar
Resistência à meticilina
Staphylococcus aureus
Abstract in Portuguese
Os objetivos do presente estudo foram avaliar os perfis de sensibilidade a antimicrobianos de MRSA isolados de sangue, identificar o tipo de SCCmec e analisar o perfil de DNA desses isolados para verificar a existência de linhagens predominantes. Amostras de MRSA isoladas de sangue foram submetidas ao teste de triagem em ágar para detecção de resistência a oxacilina, teste de sensibilidade aos antimicrobianos, PCR multiplex para detecção dos genes mecA e coa e do tipo de SCCmec, tipagem molecular por PFGE. 89% das amostras de MRSA foi de origem hospitalar. Foi observado multirresistência em 69% dos isolados. 83% dos isolados apresentaram SCCmec tipo IIIA e um perfil PFGE predominante. Foram observadas amostras de MRSA sensíveis a diversas classes de antimicrobianos, portando SCCmec tipo IV, associadas a infecção de origem hospitalar e a tipagem molecular permitiu observar o predomínio de um clone, disseminado em todo o complexo HC
Keywords in English
Cross infection
Epidemiology molecular
Methicillin resistance
Staphylococcus aureus
Abstract in English
The aims of this study were to evaluate the susceptibility profile of MRSA strains isolated from blood, identify the SCCmec types and analyze the DNA profile in order to determine if there were predominant lineages. Consecutive MRSA blood isolates were submitted to oxacillin agar screening test, antimicrobial susceptibility test, multiplex PCR to detect mecA and coa genes, SCCmec typing and molecular typing by PFGE. 89% of the isolates were nosocomial-acquired. 69% of strains were observed to be multiresistant. 83% of the 223 isolates had SCCmec type IIIA and had a predominant DNA pattern by PFGE. Some strains nosocomial-acquired had SCCmec type IV, resistance only to beta-lactams and demonstrated PFGE pattern with one predominant clone
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