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Master's Dissertation
DOI
10.11606/D.5.2016.tde-09082016-151216
Document
Author
Full name
Wellington Luiz Ferreira da Silva
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Sotto, Mirian Nacagami (President)
Fernandes, Elaine Raniero
Negro, Gilda Maria Barbaro Del
Title in Portuguese
Interação do Paracoccidioides brasiliensis com células dendríticas e queratinócitos em biopsias de lesões de pele e mucosa oral
Keywords in Portuguese
Células de Langerhans
Células dendríticas
Imuno-histoquímica
Microscopia confocal
Mucosa bucal
Paracoccidioidomicose
Pele
Queratinócitos
Abstract in Portuguese
A paracoccidioidomicose (PCM) é uma doença sistêmica causada pelos fungos Paracoccidioides brasiliensis e Paracoccidioideslutzii. O comprometimento da pele e mucosa oral são frequentes na PCM. As células dendríticas e queratinócitos do tegumento, devido à sua função como células apresentadoras de antígenos, atuam na resposta imune inata e adaptativa contra agentes patogênicos. Com o objetivo de verificar a interação do P. brasiliensis com essas células, estudamos 47 biopsias de mucosa oral e 52 de pele de lesões de doentes com diagnóstico comprovado de PCM. As biopsias foram submetidas à técnica de imuno-histoquímica de dupla-marcação com os anticorpos anti-fator XIIIa (marcador de dendrócitos dérmicos), anti-CD207 (marcador de células de Langerhans maduras), anti-pancitoqueratinas (AE1-AE3) e anti-P. brasiliensis. Fez-se também a reação de dupla marcação por técnica de imunofluorescência, com análise por microscopia confocal a laser, para a melhor visualização da interação entre queratinócitos e os fungos. Quarenta e dois por cento das amostras de mucosa oral exibiram formas fúngicas no citoplasma de dendrócitos dérmicos. As células de Langerhans, tanto nas biopsias de mucosa oral como de pele, não mostraram leveduras ou antígenos do fungo no seu citoplasma. Cinquenta e quatro por cento das biopsias de pele e sessenta por cento das amostras de mucosa exibiram leveduras no citoplasma de queratinócitos. Os resultados obtidos permitem concluir que o parasitismo de queratinócitos pode representar possível mecanismo de evasão do fungo aos mecanismos imunes locais. Os dendrócitos dérmicos fator XIIIa positivos e queratinócitos podem estar a atuar como células apresentadoras de antígenos para suprir a função, provavelmente prejudicada, das células de Langerhans nas lesões de pele e mucosa oral da PCM humana
Title in English
Paracoccidioides brasiliensis interacts with dermal dendritic cells and keratinocytes in human skin and oral mucosa lesions
Keywords in English
Confocal microscopy
Dendritic cells
Immunohistochemistry
Keratinocytes
Mouth mucosa
Paracoccidioidomycosis
Skin, Langerhans cells
Abstract in English
Paracoccidioidomycosis (PCM) is a systemic disease caused by the fungus Paracoccidioides brasiliensis, which compromises various organs, mainly the lungs. The skin and oral mucosa are often affected. Dendritic cells and keratinocytes of the integument play a role in innate and adaptive immune response against pathogens, due to their function as antigen presenting cells. Aiming to verify the interaction of P. brasiliensis with these cell populations, we studied 52 biopsies of skin and 47 oral mucosa samples taken from patients with proven diagnosis of PCM. The biopsies were subjected to double immune staining technique with anti-factor XIIIa (marker of dermal dendrocytes), anti- CD207 (marker of mature Langerhans cells), anti-pan cytokeratins (AE1-AE3) and anti P. brasiliensis antibodies. Analyses with confocal laser microscopy were also performed to better visualization of the interaction between keratinocytes and the fungi. Factor XIIIa + dermal dendrocytes of 42% samples of oral mucosa displayed yeast forms in their cytoplasm. We did not observe yeast cells in the cytoplasm of Langerhans cells in both skin and oral mucosa samples. Fifty -four percent of skin and 60% of mucosal samples displayed yeast cells in the cytoplasm of keratinocytes. The parasitism of keratinocytes may represent a possible mechanism of evasion of the fungus to local immune mechanisms, or even as a result of keratinocytes ability to antigen presentation in PCM. Factor XIIIa dendrocytes and keratinocytes may be acting as antigenpresenting cells to fulfill the function of Langerhans cells, probably impaired, in skin and mucosa of human PCM
 
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Publishing Date
2016-08-10
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • SILVA, W. L. F., PAGLIARI, C., e Sotto, M.N. Interação do Paracoccidioides brasiliensis com queratinócitos em lesões de pele e mucosa oral. In XLVII Congresso da Sociedade Brasileira de Medicina Tropical, Natal, 2011. Resumos., 2011. Resumo.
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