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Doctoral Thesis
DOI
https://doi.org/10.11606/T.46.2013.tde-16012014-080943
Document
Author
Full name
Nídia Cristiane Yoshida
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Kato, Massuo Jorge (President)
Gamboa, Ian Castro
Garcez, Walmir Silva
Miyamoto, Sayuri
Oliveira, Luciana Gonzaga de
Title in Portuguese
Análises filogenéticas e estudo do metabolismo fenilpropanoidico em espécies de Piper
Keywords in Portuguese
Microssatélite
Piper
Piper solmsianum
Piperaceae
Propenilfenol sintase
Proteína dirigente
Abstract in Portuguese
A análise filogenética e reconstrução dos caracteres químicos em espécies de Piper indicou clados especializados na produção de ácidos benzoicos/cromenos, amidas e lignóides. Os perfis químicos das espécies foram determinados pela análise dos extratos brutos por RMN de 1H combinados com análise de componente principal (PCA). Destacou-se a ocorrência de lignanas dibenzilbutirolactônicas e furofurânicas no clado Macrostachys, enquanto que para Schilleria foi observado o acúmulo preferencial de fenilpropanóides, lignanas tetraidrofurânicas e benzofurânicas. Espécies de localidades distintas classificadas como Piper solmsianum apresentaram diferentes perfis químicos e características morfológicas. A análise da variabilidade química e genética por PCA e microssatélites, respectivamente, indicou a segregação das populações de acordo com o local de coleta e apontou o potencial para o uso conjunto das técnicas para distinguir os espécimens de P. solmsianum. As sequências dos genes acetiltransferase, propenilfenol sintase e proteína dirigente forneceram as primeiras informações sobre os genes envolvidos na biossíntese de lignanas/fenilpropanoides em P. solmsianum.
Title in English
Phylogenetic analyses and phenylpropanoid metabolism in Piper species.
Keywords in English
Dirigent protein
Microsatellites
Piper
Piper solmsianum
Piperaceae
Propenylphenol synthase
Abstract in English
The phylogenetic analysis and reconstruction of chemical characters in Piper species distinguished clades specialized in the production of benzoic acids/chromenes, amides and lignoids. The chemical profiles of these species were determined by analysis of crude extracts by 1H NMR combined with principal component analysis (PCA). It is remarkable that lignans of dibenzylbutyrolactone and furofurans type occur in Macrostachys while Schilleria contains dimers of propenylphenols such as tetrahydrofurans lignans and benzofuran neolignans. Piper solmsianum specimens collected from distinct sites showed differences in their morphology and chemical profiles. The assessment of chemical and genetic variability by PCA and microsatellites, respectively, indicated the segregation of populations according to the sampling site and pointed out to the potential use for the combined approaches to distinguish P. solmsianum specimens. The sequences of the genes acetyltransferase, propenylphenol synthase and dirigent protein provided the first information about the genes involved in the biosynthesis of lignans/phenylpropanoids in P. solmsianum.
 
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Publishing Date
2014-03-20
 
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