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Master's Dissertation
DOI
10.11606/D.46.2002.tde-21122015-154357
Document
Author
Full name
Glauber da Costa de Brito
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2002
Supervisor
Committee
Tanizaki, Martha Massako (President)
Carmona-Ribeiro, Ana Maria
Sampaio, Magda Maria Sales Carneiro
Title in Portuguese
Avaliação imunológica de vacina de polissacarídeo meningocócico C conjugado e encapsulado em lipossomo
Keywords in Portuguese
Conjugação
Imuno-hematologia
Lipossomo
Meningite viral
Polissacarídeos (Aplicações terapêuticas)
Tétano
Vacinas (Avaliação)
Abstract in Portuguese
A tecnologia de conjugação melhorou a imunogeniddade de vacinas constituídas de polissacarídeo, especialmente em crianças. Polissacarídeos conjugados a proteínas carregadoras, como o toxóide tetânico, induzem resposta imunológica dependente de célula T e memória imunológica de longa duração. No entanto, esta tecnologia apresenta custo elevado. Assim, foi investigada a capaddade dos lipossomos de aumentar a resposta imunológica ao polissacarídeo C de Neisseria meningitidis (PSC). Camundongos foram imunizados com Iipossomos contendo PSC, conjugado toxóide tetânico-PSC ou PSC livre como controle, com dose de reforço constituída de PSC livre. Foram gerados anticorpos IgG e IgM contra PSC nos camundongos imunizados com conjugado ou Iipossomo. Os resultados mostram que lipossomos contendo PSC têm potencial para substituir a vadna conjugada toxóide tetânico-PSC.
Title in English
Immunological evaluation of meningococcal polysaccharide C conjugate vaccine and encapsulated liposome
Keywords in English
Conjugation
Immuno-hematology
Liposome
Polysaccharides (Therapeutic applications)
Tetanus
Vaccines (Evaluation)
Viral meningitis
Abstract in English
Conjugation technology has improved the immunogenicity of polysaccharide vaccines, specially in small children. Polysaccharides conjugated to various carrier proteins, e.g. tetanus toxoid, stimulate a T cell-dependent antibody response and induce a long-term immunological memory. However, protein-polysaccharide conjugation technology is expensive and this could constitute an important drawback. Thus, immunopotentiation of Neisseria meningitidis serogroup C polysaccharide (PSC) by use of liposomes as an alternative to protein-polysaccharide C conjugates was investigated. Mice were immunized with liposomes containing PSC or tetanus toxoid-PSC conjugate or free PSC as control and boosted with free PSC. Immunogenicity of these different preparations was compared with each other. Conjugate and liposome containing PSC induced both IgG and IgM antibodies against the polysaccharide. These results show that liposomes containing entrapped PSC have potential to be used as an alternative to tetanus toxoid-PSC conjugate vaccine.
 
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Publishing Date
2015-12-21
 
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