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Master's Dissertation
DOI
https://doi.org/10.11606/D.43.2020.tde-15022021-145804
Document
Author
Full name
Ricardo Rovere de Santi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2020
Supervisor
Committee
Alencar, Adriano Mesquita (President)
Brito, Isis Vasconcelos de
Moriya, Henrique Takachi
Title in Portuguese
Montagem de uma pinça óptica e sua calibração: Estudo teórico e simulação computacional de sua força
Keywords in Portuguese
GLMT
Pinça óptica
simulação computacional de força óptica no regime de Lorenz - Mie
Abstract in Portuguese
A pinça óptica tem se tornado uma importante ferramenta na análise de sistemas biológicos, podendo ser utilizada, desde o estudo de moléculas de DNA, ao estudo de micro-organismos, permitindo que sejam medidas as suas propriedades físicas e reológicas, ou manipulando sua posição para estudar seu comportamento. O presente projeto de pesquisa consistiu em montar um aparato de pinça óptica para aplicar em técnicas microscópicas, tais como microrreologia. Estudamos o fundamento teórico da pinça e realizamosa análise numérica através de simulações da força no regime de Lorenz - Mie. Esse trabalho permitirá a adaptação de uma pinça óptica ao sistema de Microscopia de Força e Tração (TFM), para o estudo mais amplo de propriedades mecânicas de células vivas.
Title in English
Assembly of an optical tweezrs and its calibration: theoretical study and computational simulation of its strength
Keywords in English
Computer simulation of optical force in the Lorenz - Mie regime
GLMT
optical tweezer
Abstract in English
Optical tweezers have become an important tool in the analysis of biological systems, and can be used from the study of DNA and molecules to the study of microorganisms, allowing their physical and rheological properties to be measured, or manipulating their position to study their behavior. The present research project consisted of mounting an optical tweezers apparatus to applyonmicroscopic techniques, such as microrheology. It was intended to study the theoretical basis of the tweezers and to perform the numerical analysis through simulations of the force under the Lorenz-Mie regime. This work will allow the construction of an optical tweezers coupled to TractionForce Microscopy (TFM) in the future, for the broader study of mechanical properties of living cells.
 
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disertacao.pdf (4.69 Mbytes)
Publishing Date
2021-02-23
 
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