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Master's Dissertation
DOI
10.11606/D.42.2011.tde-23012012-154044
Document
Author
Full name
Daniel Simões de Jesus
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Carpinelli, Angelo Rafael (President)
Anhê, Gabriel Forato
Cipolla Neto, Jose
Title in Portuguese
A influência da pinealectomia na funcionalidade das células beta pancreáticas.
Keywords in Portuguese
Ativação enzimática
Glândula pineal
Ilhotas de Langerhans
Insulina
Melatonina
Secreção
Abstract in Portuguese
As ilhotas pancreáticas têm em sua constituição as células b pancreáticas, as quais têm como função secretar insulina. A melatonina é secretada pela glândula pineal. No entanto, a ausência da melatonina, por meio da pinealectomia (PINX), induz diversas alterações nas funções celulares. A NAD(P)H oxidase é responsável pela produção de ânions superóxido. Nosso estudo teve como objetivo avaliar uma possível modulação da NAD(P)H oxidase pela PINX no Zeitgeber Time 6 e 18. Os resultados demonstram que a PINX induz alterações na funcionalidade das ilhotas pancreáticas, alterando a secreção de insulina estimulada pela glicose, o metabolismo da glicose, e o conteúdo de espécies reativas de oxigênio (EROs). Entretanto, não induz alterações nos respectivos ZTs na expressão protéica das subunidades p22phox, p47phox e gp91phox. Demonstramos que a pinealectomia induz alteração no padrão rítmico metabólico e secretório nas ilhotas isoladas, tendo a NAD(P)H oxidase uma possível participação no desenvolvimento nas alterações observadas.
Title in English
The influence of pinealectomy in pancreatic beta cell functionality.
Keywords in English
Enzyme activation
Insulin
Islets of Langerhans
Melatonin
Pineal gland
Secretion
Abstract in English
Pancreatic islets are constituted by pancreatic cells, which main function is to secrete insulin. Melatonin is secreted by the pineal gland. However, the absence of melatonin by pinealectomy (PINX) induces several changes in cellular functions. The NAD(P)H oxidase is responsible for producing superoxide. Our study aimed to evaluate the possible modulation of NAD(P)H oxidase by pinealectomy in Zeitgeber Time 6:18. Our results show that the absence of melatonin induced by pinealectomy induces changes in the functionality of pancreatic islets, such as the insulin secretion stimulated by glucose, the glucose metabolism, and the content of reactive oxygen species (ROS). However, it does not induce changes in their respective Zts in the protein expression of the subunits p22phox, p47phox and gp91phox. We demonstrated that pinealectomy induces changes in metabolic and secretory rhythm pattern in isolated islets, being the NAD(P)H oxidase a possibly responsible for the changes observed.
 
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Publishing Date
2012-02-13
 
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