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Doctoral Thesis
DOI
10.11606/T.42.2012.tde-18092012-080950
Document
Author
Full name
Claudia Ferreira dos Santos Ruiz Figueiredo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Souza, Maria Oliveira de (President)
Aires, Margarida de Mello
Dellova, Deise Carla Almeida Leite
Gomes, Guiomar Nascimento
Magaldi, Antonio Jose Barros
Title in Portuguese
Efeito da espironolactona sobre a função e morfologia renal de ratos hipertensos por infusão crônica de Angiotensina II.
Keywords in Portuguese
Aldosterona
Angiotensina II
Eletrólitos
Função renal de animal
Hipertensão
Renina
Abstract in Portuguese
Este estudo tem o objetivo de investigar a participação da espironolactona, um antagonista dos receptores MR, sobre as respostas renais induzidas pela hiperatividade do SRAA. Utilizamos 2 grupos de animais tratados com Ang II ( 15 e 28 dias). Foram avaliados o fluxo plasmático renal (FPR) e Ritmo de filtração glomerular (RFG), eletrólitos no plasma e urina, expressão proteica e morfologia renal. Nossos resultados indicam que, a Ang II (15 e 28 dias), induziu à hipertensão, queda do FPR, RFG, fluxo urinário, e aumentou a fração de filtração (FF), a expressão de NHE3, a área glomerular e alterações morfológicas no túbulo proximal, incluindo perda da borda em escova e morte celular. A espironolactona corrigiu a maioria dos efeitos da Ang II. A Ang II e/ou espironolactona não modificaram a carga excretada de NH4, Na+ ou K+. Os resultados indicam que a hiperatividade do SRAA induz aumento na concentração plasmática de aldosterona, e esta contribui para os efeitos deletérios do SRAA sobre a função e morfologia renal.
Title in English
Effect of spironolactone on renal function and morphology of hypertensive rats by Ang. II chronic infusion.
Keywords in English
Aldosterone
Angiotensin II
Electrolytes
Hypertension
Renal function of animal
Renin
Abstract in English
This study investigated the effect of spironolactone (MR antagonist) on renal function and morphology in hypertensive rats with chronic Ang II infusion for 15 or 28 days. Blood pressure (BP) was monitored. Renal plasmatic flow (RPF) and glomerular filtration rate (GFR) were determined. Plasmatic levels of renin and aldosterone, urinary excretion of ions, protein expression and renal morphology were analyzed. Our results showed that Ang II used was enough to induce hypertension in both periods. The RPF, GFR and urinary flux decreased. Ang II increased the filtration fraction (FF). Spironolactone impaired all effect of Ang II. The plasma renin and aldosterone levels increased and spironolactone amplified both. Urinary Na+ and K+ did not changed in Ang II and/or spironolactone rats treated. Ang II increased the NHE3 expression, induced proximal tubule injury and cellular apoptosis. Spironolactone impaired the effect of Ang II. In conclusion, spironolactone has a beneficial effect on renal injury induced by hyperactivity of renin-angiotensin-aldosterone system.
 
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Publishing Date
2012-10-19
 
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