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Master's Dissertation
DOI
10.11606/D.42.2014.tde-11122014-111724
Document
Author
Full name
Caio Jordão Teixeira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Carvalho, Carla Roberta de Oliveira (President)
Bazotte, Roberto Barbosa
Lima, Fabio Bessa
Title in Portuguese
Gonadectomia e DHEA: efeitos metabólicos em ratas adultas alimentadas com dieta hiperlipídica.
Keywords in Portuguese
Castração
Deidroepiandrosterona
Menopausa
Obesidade
Ovariectomia
Resistência à insulina
Abstract in Portuguese
A privação dos hormônios sexuais em fêmeas contribui para o aparecimento de distúrbios metabólicos e endócrinos devido à ausência de estrógenos. Nesse estudo foi avaliado o efeito da castração e da suplementação com DHEA sobre aspectos metabólicos em ratas. A ovariectomia aumentou a adiposidade e o consumo de ração, diminuiu o consumo hídrico, além de promover resistência à insulina e intolerância à glicose, e o uso do DHEA reduziu a sensibilidade à insulina. Ainda, a castração levou a redução nos níveis plasmáticos de DHEA, estradiol, VLDL-c e triacilglicerol, albumina, uréia e creatinina, e o tratamento com DHEA restaurou parcialmente a concentração de estradiol no grupo OVX+DHEA. E por fim, a ooforectomia reduziu a expressão proteica do IRb, IRS1, PI3K e AKT no fígado, músculo gastrocnêmio ou coração, além diminuir o grau de fosforilação em tirosina da pp185 (IRS1/2). Deste modo, concluímos que a castração promoveu resistência à insulina e intolerância à glicose associada à obesidade, e neste modelo, o uso do DHEA piorou a sensibilidade à insulina nos animais.
Title in English
Gonadectomy and DHEA: metabolic effects in adult female rats fed with high-fat diet.
Keywords in English
Castration
Dehydroepiandrosterone
Insulin resistance
Menopause
Obesity
Ovariectomy
Abstract in English
Deprivation of sex hormones in females contributes to the onset of metabolic and endocrine disorders due loss of estrogen. In this study the effect of castration and supplementation with DHEA on metabolic aspects in rats was evaluated. Ovariectomy increased adiposity and food consumption, decreased water consumption, besides promoting insulin resistance and glucose intolerance, and the use of DHEA reduced insulin sensitivity. Furthermore, castration led to decreasing of DHEA plasma levels, estradiol, VLDL-c and triacylglycerol, albumin, urea and creatinine, and DHEA treatment partially restored the concentration of estradiol in OVX+DHEA group. Finally, ovariectomy reduced the protein expression IRb, IRS1, PI3K and AKT in the liver, gastrocnemius muscle or heart. Indeed, decrease tyrosine phosphorylation status of pp185 (IRS1/2) was observed. Thus, we conclude that castration promoted insulin resistance and glucose intolerance associated with obesity, and in this model, the use of DHEA impairs insulin sensitivity in animals.
 
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Release Date
2018-12-15
Publishing Date
2014-12-16
 
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