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Master's Dissertation
DOI
10.11606/D.42.2016.tde-10082016-090534
Document
Author
Full name
Kelly Priscila Pandolfi dos Santos
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Aires, Margarida de Mello (President)
Helou, Claudia Maria de Barros
Onuchic, Luiz Fernando
Title in Portuguese
Efeitos da aldosterona sobre a expressão proteica do EGFR e ERK1/2 fosforilados e o desenvolvimento de lesões no rim de ratos.
Keywords in Portuguese
Aldo
Efeito genômico
EGFR
ERK1/2
Injúrias renais
Abstract in Portuguese
O objetivo desse estudo foi avaliar o efeito do tratamento com a aldosterona (Aldo), por 21 dias, sobre a função e a morfologia renal de ratos, procurando correlacionar as alterações com a expressão do EGFR e da ERK1/2 fosforilados. A Aldo não alterou os parâmetros fisiológicos, a PA e a função renal dos ratos, mas verificou-se uma tendência para o aumento das concentrações plasmáticas de K+. No córtex e na medula renal, a Aldo aumentou a expressão do EGFR e da ERK1/2 fosforilados, sendo que este efeito foi abolido pelo tratamento concomitante com a espironolactona ou o RU 486 (antagonistas dos receptores da Aldo). O tratamento hormonal também induziu um aumento na marcação imuno-histoquímica para essas proteínas no córtex e medula; contudo, este efeito foi reduzido, principalmente, com o tratamento conjunto com a espironolactona. Observou-se um aumento na área glomerular e na porcentagem da área intersticial, após o tratamento com a Aldo. As alterações na morfometria renal foram parcialmente reduzidas pelo tratamento com a espironolactona ou o RU 486. Esses resultados indicam que a Aldo pode induzir alterações morfológicas no rim, aparentemente, de maneira dependente dos receptores MR e GR e da via do EGFR-ERK1/2, mas sem o comprometimento da função renal.
Title in English
Aldosterone effects on the protein expression of phosphorylated EGFR and ERK 1/2 and development of lesions in the rat kidney.
Keywords in English
ALDO
EGFR
ERK 1/2
Genomic effect
Kidney injuries
Abstract in English
The purpose of this study was to evaluate the effect of 21 days of treatment with aldosterone (Aldo) in the renal function and morphology of rats, correlating changes with the expression of EGFR and ERK1/2 phosphorylated. The Aldo did not alter physiological parameters, blood pressure and renal function of rats, but there was a tendency to increased K+ plasma concentrations. In renal cortex and medulla, Aldo increased the expression of EGFR and ERK1/2 phosphorylated and this effect was abolished by treatment Aldo plus spironolactone or RU 486 (Aldo receptor antagonists). Hormonal treatment also induced an increase in immunohistochemical staining for these proteins in the cortex and medulla; however, this effect was limited mainly to treatment Aldo plus spironolactone. There was an increase in glomerular area and the percentage of interstitial area after treatment with Aldo. Changes in renal morphology were partially reduced by treatment Aldo plus spironolactone or RU 486. These results indicate that Aldo may induce morphological changes in the kidney of dependent manner of MR and GR receptors and EGFR-ERK1/2 signaling, but without the impairment of renal function.
 
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Release Date
2018-08-10
Publishing Date
2016-08-10
 
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