• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2018.tde-05022018-151218
Document
Author
Full name
Jéssica Santiago da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Souza, Maria Oliveira de (President)
Dellova, Deise Carla Almeida Leite
Machado, Ubiratan Fabres
Nishi, Erika Emy
Title in Portuguese
Efeito da endotelina 1 na atividade do trocador Na+/H+ em células do túbulo proximal renal.
Keywords in Portuguese
Endotelina 1
Trocador Na+/H+
Túbulo proximal renal
Abstract in Portuguese
O rim é tanto um órgão-alvo como a principal fonte de produção de ET-1, peptídio que regula a excreção de Na+ e água por este órgão que expressa os seus respectivos receptores, ETA e ETB, além dos trocadores NHE1 e NHE3 que são essenciais para o equilíbrio ácido base e hidroeletrolítico das células. Assim, o objetivo deste estudo foi investigar, em células IRPTC, o papel de ET-1 na atividade dos trocadores NHE1 e NHE3. Nossos resultados indicam que o tratamento agudo com ET-1 (10-9 M) aumenta a velocidade de recuperação do pHi (dpHi/dt) nos dois primeiros minutos após o pulso ácido, sugerindo aumento na atividade dos trocadores NHE1 e NHE3, que ocorre via ativação dos receptores ETA e ETB e parece ser secundária à atividade da p90RSk e p38MAPK. O tratamento crônico com ET-1 (10-9 M) reduz a dpHi/dt nos dois primeiros minutos após o pulso ácido, o que sugere redução na atividade dos trocadores NHE1 e NHE3, que pode ser secundária à inibição da Na+, K+-ATPase por ET-1.
Title in English
Effectofendothelin 1 on Na+/H+ exchangeractivity in renal proximal tubulecells.
Keywords in English
Endothelin 1
Na+/H+ exchanger
Renal proximal tubule
Abstract in English
The kidney is both a target organ and the main source of ET-1 production, a peptide that regulates the excretion of Na+ and water by this organ that expresses its respective receptors, ETA and ETB, in addition to the NHE1 and NHE3 exchanger which are Essential for the basic acid and electrolyte balance of cells. Thus, the objective of this study was to investigate, in IRPTC cells, the role of ET-1 in the activity of the NHE1 and NHE3 exchanger. Our results indicate that the acute treatment with ET-1 (10-9 M) increases the rate of recovery of pHi (dpHi/dt) in the first two minutes after the acid pulse, suggesting an increase in the activity of the NHE1 and NHE3 exchanger, which occurs via activation Of ETA and ETB receptors and appears to be secondary to the activity of p90RSk and p38MAPK. Chronic treatment with ET-1 (10-9 M) reduces dpHi/dt in the first two minutes after the acid pulse, suggesting a reduction in NHE1 and NHE3 exchanger activity, which may be secondary to inhibition of Na+, K+- ATPase by ET-1.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Release Date
2020-02-05
Publishing Date
2018-02-05
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.