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Master's Dissertation
DOI
10.11606/D.42.2014.tde-20022015-131713
Document
Author
Full name
Antonio Garcia Soares Júnior
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Akamine, Eliana Hiromi (President)
Ceravolo, Graziela Scalianti
Muscara, Marcelo Nicolas
Title in Portuguese
Obesidade induz alterações artéria-específica: avaliação da função endotelial e do fenótipo das células musculares lisas.
Keywords in Portuguese
Endotélio vascular
Músculo liso vascular
Obesidade
Óxido nítrico
Abstract in Portuguese
A obesidade pode mudar as características das células endoteliais e musculares lisas (CMLVs). Reatividade e genes inflamatórios e de marcadores de fenótipo de CMLVs foram avaliados em artérias mesentéricas de resistência (AMRs) e de aorta de camundongos alimentados com dieta hiperlipídica (OB). RNAm para citocinas pró-inflamatórias e IL-10 foi aumentado em AMRs e aorta de OB. O relaxamento não foi alterado, mas a contração foi reduzida em AMRs e aorta de OB. AMRs apresentaram redução global da contração e a aorta apresentou redução específica para agonista adrenérgico. Maior modulação negativa por NO e prostanoides vasodilatadores foi observada em aorta, mas não em AMRs. RNAm para marcador do fenótipo sintético foi aumentado em AMRs de OB. Esses resultados mostram que as células endoteliais e as CMLVs de AMRs e aorta respondem diferentemente à obesidade. Inflamação e mecanismo de contrarregulação são induzidos em AMRs e aorta, que impediria a disfunção endotelial, mas não a mudança fenotípica das CMLVs em AMRs, que então, comprometeria sua capacidade contrátil.
Title in English
Obesity leads to artery-specific alterations: evaluation of the endothelial function and smooth muscle cell phenotype.
Keywords in English
Nitric oxide
Obesity
Vascular endothelium
Vascular smooth muscle
Abstract in English
Obesity may change the vascular endothelial cells and smooth muscle cells (VSMC) characteristics. Vascular reactivity and inflammatory and phenotypic markers for VSMC gene expression in resistance mesenteric arteries (RMA) and aorta from mice fed with high fat diet (OB). Pro-inflammatory cytokines mRNA and IL-10 were elevated in OB's RMA and aorta. The relaxation wasnt altered, however a reduction in contractility was observed in OB's RMA and aorta. A global reduction in contractility was observed in RMA and aorta demonstrated a specific reduction to adrenergic agonist. Higher negative modulation by NO and vasodilator prostanoids were seen only in aorta. Phenotypic markers mRNA were elevated in OB's RMA. The results shows that endothelial cells and VSMC from RMA and aorta respond differently to obesity. Inflammation and counter regulatory mechanisms are induced in RMA and aorta of which would prevent endothelial dysfunction but not VSMC phenotypic changing from RMA, compromising the contractile ability.
 
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Release Date
2017-02-19
Publishing Date
2015-02-20
 
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