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Doctoral Thesis
DOI
10.11606/T.42.2012.tde-13112012-114518
Document
Author
Full name
Fernando Paranaiba Filgueira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2012
Supervisor
Committee
Carvalho, Maria Helena Catelli de (President)
Akamine, Eliana Hiromi
Dantas, Ana Paula Villela
Franco, Maria do Carmo Pinho
Lacchini, Silvia
Title in Portuguese
Caracterização da resposta vasorelaxante do equilin em artérias mesentéricas de ratas espontaneamente hipertensas.
Keywords in Portuguese
Endotélio
Estrógenos
Hipertensão
Ratos
Sistema renina-angiotensina
Abstract in Portuguese
Este estudo investigou a ação do equilin em artérias mesentéricas de resistência de ratas espontaneamente hipertensas, bem como o mecanismo envolvido, comparando com o 17b-estradiol. O equilin promoveu vasodilatação equivalente à do 17b-estradiol, não demonstrando diferença nas respostas observadas em ratas intactas e ovariectomizadas. A resposta ao equilin não foi alterada pelo antagonista de receptores de estrógeno. De modo similar, a remoção do endotélio ou a inibição da adenilato ciclase, da PKA, da óxido nítrico sintase, da guanilato ciclase e da PKG não afetou o relaxamento ao equilin. Além disso, a incubação com diferentes bloqueadores de canais de K+ não alterou o relaxamento ao equilin. O equilin diminuiu a contração ao CaCl2 e ao BAYK 8644 (ativador de canais de Ca2+ do tipo-L), porém, não modificou a contração à cafeína (que promove liberação de Ca2+ do retículo sarcoplasmático), demonstrando que o efeito relaxante do equilin em artérias mesentéricas de ratas espontaneamente hipertensas se deve predominantemente ao bloqueio de canais de Ca2+ do tipo L.
Title in English
Characterization of vasorelaxant response to equilin in mesenteric arteries from spontaneously hypertensive rats.
Keywords in English
Endothelium
Estrogens
Hypertension
Rats
Renin-angiotensin system
Abstract in English
The present study investigated the action of equilin in mesenteric resistance arteries from female hypertensive rats. Mechanisms contributing to equilin-induced effects were determined, comparing with 17b-estradiol. Equilin evoked vasodilatation equivalent to that of 17b-estradiol, with no difference between intact and ovariectomized rats. Equilin-induced response was not altered by the estrogen receptor antagonist. Similarly, endothelium removal or inhibition of adenylyl cyclase, PKA, NOS, guanylate cyclase or PKG did not affect the relaxation to equilin. Furthermore, the relaxation to this hormone was not altered after incubation with K+ channel blockers. Equilin reduced contraction induced by both CaCl2 and Bay K 8644 (an L-type Ca2+ channel activator), however, it was unable to alter caffeine-induced contraction (via Ca2+ release from the intracellular stores), demonstrating that equilin vasorelaxant effect in mesenteric arteries from female spontaneously hypertensive rats occurs predominantly due to blockade of L-type Ca2+ channels.
 
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Release Date
2017-12-21
Publishing Date
2013-02-08
 
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