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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2014.tde-24022015-162835
Document
Author
Full name
Zuleima Del Carmen Caballero Espinosa
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Teixeira, Marta Maria Geraldes (President)
Lima, Maria Regina D'Imperio
Padrón, Thais Cristina Baeta Soares Souto
Silveira Filho, José Franco da
Zingales, Bianca Silvana
Title in Portuguese
Origem, evolução e relações filogenéticas de homólogos de prolina racemase em espécies de Trypanosoma.
Keywords in Portuguese
Trypanosoma
Filogenia
PRAC
Prolina racemase
Transferência horizontal de genes
Abstract in Portuguese
Os genes de Prolina racemase (PRACs) são enzimas intracelulares ou secretadas de T. cruzi (TcPRAC); essas enzimas estão envolvidas no metabolismo, diferenciação e virulência. Os genes PRAC foram identificados e caracterizados molecularmente em isolados representantes de toda a diversidade interespecífica de T. cruzi, T. cruzi marinkellei, T. dionisii, T. erneyi, T. rangeli, T. conorhini e T. lewisi. Além dessas espécies de tripanossomas restritas a mamíferos; homólogos de PRAC foram encontrados em tripanossomas de cobra (T. serpentis), crocodilo (T. grayi) e anuro (T. sp. 339). Análises filogenéticas e de sintenia entre homólogos de PRAC suportaram uma historia evolutiva totalmente congruente com as relações evolutivas previamente descritas dentro do gênero Trypanosoma.
Title in English
Origin, evolution and phylogenetic relationships of proline racemase homologs from species of Trypanosoma.
Keywords in English
Trypanosoma
Horizontal gene transfer
Phylogeny
PRAC
Proline racemase
Abstract in English
Proline racemaces (PRACs) are intracellular or secreted enzymes of Trypanosoma cruzi (TcPRAC), implicated in metabolism, differentiation, virulence and the induction of nonspecific polyclonal B-lymphocyte in the host. We identified and molecularly characterized PRAC genes from isolates representing all intra-specific diversity (TcI-TcVI and Tcbat), T. cruzi marinkellei, T. dionisii, T. erneyi, T. rangeli (isolates of lineages A-E), T. conorhini, and T. lewisi. In addition to these trypanosome species restricted to mammals, PRAC homologs were found in trypanosomes from snake (T. serpentis), crocodile (T. grayi) and anuran (T. sp 339). Phylogenetic and synteny analysis of PRAC homologs supported an evolutionary history totally congruent with the evolutionary relationships within the genus Trypanosoma.
 
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Publishing Date
2015-02-24
 
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