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Master's Dissertation
DOI
10.11606/D.42.2014.tde-18072014-141833
Document
Author
Full name
Danielle da Silva Menchaca Vega
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Katzin, Alejandro Miguel (President)
Fischer, Dominique Corinne Hermine
Toledo, Erika Suzuki de
Title in Portuguese
Caracterização de fitol e verificação de uma segunda via de biossíntese de filoquinona e tocoferol nos estágios intraeritrocíticos de Plasmodium falciparum.
Keywords in Portuguese
Plasmodium falciparum
Fitol
Malária
Vitamina E e K1
Abstract in Portuguese
Nosso grupo tem caracterizando diversos produtos da via de isoprenóides em Plasmodium falciparum que é promissora para o desenvolvimento de novas drogas. Dentre os compostos isoprenicos identificados estão as vitaminas K1 e E que apresentam uma cadeia isoprenica fitil. Em plantas o fitol é originado da degradação da clorofila. mas, como ainda não existem evidências da presença de clorofila no parasito, sugerimos que este possa ser formado pela degradação dessas vitaminas. Parte do fitol seria ligado a ácidos graxos utilizado como constituinte de membrana, e parte sofreria fosforilações, formando as vitaminas E e K1 como uma segunda via de biossíntese desse composto, assim como ocorre em Arabidopsis thaliana. Analises por HPLC e TLC utilizando o marcador [3H]GGPP, confirmaram a presença de fitol nos estágios intraeritrocíticos. Além disso, mostramos que o [3H]-fitol pode ser convertido em fitil-P e fitil-PP por uma atividade fitol quinase utilizando nucleotídeos como doador fosfato.
Title in English
Characterization of phyto and an alternative pathway for phylloquinone and tocopherol biosynthesis in intraerythrocytic stages of Plasmodium falciparum.
Keywords in English
Plasmodium falciparum
Malaria
Phytol
Vitamin E and K1
Abstract in English
Our group has characterized differents products from isoprenoid pathway in Plasmodium falciparum which is promising for the development of new drugs. Among the isoprenics compounds identified there are the vitamins K1 and E which have a isoprenic phytyl side chain . In plants the phytol is obtained from the chlorophyll degradation. Until now theres no evidence that support its presence in the paraste. Our hypothesis that is phytol can be produced by the degradation of these vitamins E and K1. In the parasite phytol would fulfill to functions: used as a constituent of membrane, bounded to fatty acids and; be converted through phosphorylations vitamins E and K1 as a second biosynthetic pathway as well as in Arabidopsis thaliana. Analisys through and HPLC and TLC with radioactive [3H]GGPP confirmed the presence of phytol in intraerythrocytic stages. In addition, we showed that [3H]-phytol is converted into phytyl-P and phytyl-PP through a phytol kinasey using nucleotides as Phosphate donor.
 
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Release Date
2018-09-29
Publishing Date
2014-10-01
 
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